Human XBP1 (Unspliced) ELISA Kit (ab289904)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.037 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human XBP1 (Unspliced) ELISA Kit
XBP1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Extract 8 9.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Extract 3 9.5% -
サンプルの種類
Cell culture extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
>= 3.037 pg/ml -
検出範囲
9.375 pg/ml - 600 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture extracts 86 62% - 115% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human XBP1 (Unspliced) ELISA Kit (ab289904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human XBP1 (Unspliced) in cell culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human XBP1 (Unspliced) with 3.037 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human XBP1 (Unspliced) Capture Antibody 1 x 600µl 10X Human XBP1 (Unspliced) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Human XBP1 (Unspliced) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. -
関連疾患
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. -
配列類似性
Belongs to the bZIP family.
Contains 1 bZIP domain. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- Tax responsive element binding protein 5
- Tax-responsive element-binding protein 5
- TREB5
see all -
参照データベース
- Entrez Gene: 7494 Human
- Omim: 194355 Human
- SwissProt: P17861 Human
- Unigene: 437638 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289904の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of human XBP1 (Unspliced) standard curve in 1X Cell Extraction Buffer PTR.
Example of human XBP1 (Unspliced) standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked human XBP1 (Unspliced) in T-47D Cell Extract.Recombinant XBP1 (Unspliced) was spiked into the following human samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 1,000 µg/mL T-47D Cell Extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Human XBP1 (Unspliced) ELISA Kit (ab289904) -
Human XBP1 (Unspliced) ELISA Kit (ab289904)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab289904 は論文での使用が確認できていません。