AB233625

Human VEGF-D ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human VEGF-D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VEGF-D with a sensitivity of 75.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor

4 Images

Sandwich ELISA - Human VEGF-D ELISA Kit (AB233625)

sELISA

Supplier Data

Sandwich ELISA - Human VEGF-D ELISA Kit (AB233625)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,193 pg/mL with a range of 956.9 – 3,270 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human VEGF-D ELISA Kit (AB233625)

Interpolated concentrations of spike VEGF-D in human serum, plasma, and cell culture media samples.

The concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum neat, plasma (citrate) neat, plasma (heparin) neat, and RPMI cell culture media containing 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).

sELISA

Supplier Data

Sandwich ELISA - Human VEGF-D ELISA Kit (AB233625)

Interpolated concentrations of native VEGF-D in human serum and plasma samples.

The concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum neat, plasma (citrate) neat, and plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,105 pg/mL in neat serum, 1,349 pg/mL in neat plasma (citrate) and 1,232 pg/mL in neat plasma (heparin). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).

sELISA

Supplier Data

Sandwich ELISA - Human VEGF-D ELISA Kit (AB233625)

Example of human VEGF-D standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 75.7 pg/mL

検出範囲

348.2 - 10000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human VEGF-D ELISA Kit (ab233625) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF-D protein in hep plasma, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF-D with 75.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Inter", "sample": "VEGF-D/serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "VEGF-D/serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

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回収率

[ { "sample": "Serum", "range": "85 - 92 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "74 - 82 %", "average": "= 78" }, { "sample": "Citrate plasma", "range": "84 - 92 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "98 - 106 %", "average": "= 101" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.

Biological function summary

Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.

Pathways

The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.

Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.

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製品プロトコール

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ターゲットの情報

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

See full target information VEGFD

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Aging 14:4137-4157 PubMed35537818

2022

LncRNA CRART16/miR-122-5p/FOS axis promotes angiogenesis of gastric cancer by upregulating VEGFD expression.

Applications

Unspecified application

Species

Unspecified reactive species

Junling Zhang,Xiaocong Pang,Lili Lei,Jixin Zhang,Xiaoqian Zhang,Ziyi Chen,Jing Zhu,Yong Jiang,Guowei Chen,Yingchao Wu,Tao Wu,Yisheng Pan,Yucun Liu,Yimin Cui,Xin Wang

Cell death & disease 13:272 PubMed35347106

2022

Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Dong Wang,Xiao-Jun Yu,Ji-Chun Hou,Bo Fu,Hao Zheng,Qi-Kun Liu,Shan-Xi Wang,Zheng-Gang Bi,Yang Cao

Experimental and therapeutic medicine 20:141 PubMed33093879

2020

Expression and predictive value of HIF-1α and VEGF in patients with burns following treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhufeng Liu,Ling Liu,Xuejuan Cheng,Liming Gao

View all publications

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Human VEGF-D ELISA Kit