AB223591

Human VCAM1 ELISA Kit

Recombinant
SimpleStep
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(13 Publications)

Human VCAM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VCAM1 with a sensitivity of 37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

別名を表示する

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

6 Images

ELISA - Human VCAM1 ELISA Kit (AB223591)

ELISA

Supplier Data

ELISA - Human VCAM1 ELISA Kit (AB223591)

sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Example of human VCAM1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Interpolated concentrations of native VCAM1 in human serum and plasma, and interpolated concentrations of spiked VCAM1 in cell culture supernatant samples.

The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, plasma (heparin) 2.5%, and RPMI media 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 1196 ng/mL in neat serum, 1092 ng/mL in neat plasma (citrate), 1275 ng/mL in neat plasma (EDTA), and 976 ng/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 1007 ng/mL with a range of 690 – 1501 ng/mL.

sELISA

Unknown

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Human VCAM1 Competitor standard curve comparison.

Standard curve comparison between Human VCAM1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Representative image of Human VCAM1 ELISA Kit (ab223591)

Components shown from left to right :

- Sample diluent NS

- 10X Human VCAM1 Detector Antibody

- Human VCAM1 Lyophilised Purified Protein (2 vials)

- Human VCAM1 Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 37 pg/mL

検出範囲

0.14 - 9 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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製品の詳細

Human VCAM1 ELISA Kit ab223591 is a rapid single-wash 90-min Sandwich ELISA to measure Human VCAM1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 37 pg/mL.

How the assay works

Human VCAM1 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human VCAM1 ELISA Kit ab223591 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-VCAM1 antibody [EPR20348-122] - BSA and Azide free (Capture) ab244923
- Anti-VCAM1 antibody [EPR20347-57] - BSA and Azide free (Detector) ab244923

Vascular cell adhesion molecule 1 (VCAM1) is a type I transmembrane glycoprotein that facilitates the adhesion of lymphocytes, eosinophils, monocytes and basophils to vascular endothelium. VCAM1 is primarily expressed by endothelial cells, but smooth muscle cells, macrophages, oocytes, Sertoli cells, and activated neurons are all known expressers. VCAM1 expression is stimulated by cytokines, including TNF- a and IL-1, and alternatively spliced forms of the protein are known to occur. VCAM1 may be involved in cardiovascular development, atherosclerosis and rheumatoid arthritis.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.23" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]

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回収率

[ { "sample": "Serum", "range": "88 - 104 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "88 - 109 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "89 - 103 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "92 - 102 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "92 - 108 %", "average": "= 101" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

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製品プロトコール

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ターゲットの情報

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).

See full target information VCAM1

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40429851

2025

Effects of , Ohwi, and Extract on Vascular Endothelial Dysfunctions in Ovariectomized Rats and Molecular Mechanisms.

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Joohee Oh,Minseo Kim,Jinsoo Kim,Jiwon Jang,Dongjin Noh,Hyun-Sook Kim

CNS neuroscience & therapeutics 30:e70173 PubMed39727329

2024

Annexin A1 Mitigates Blood-Brain Barrier Disruption in a Sepsis-Associated Encephalopathy Model by Enhancing the Expression of Occludin and Zonula Occludens-1 (ZO-1).

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Yao Li,Fang Zhou,Jiyue You,Xinran Gong

Biomolecules & biomedicine 25:682-692 PubMed39319864

2024

Regulatory role and molecular mechanism of METTL14 in vascular endothelial cell injury in preeclampsia.

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Huafang Wei,Lin Liang,Chengwen Song,Ming Tong,Xiang Xu

Journal of thoracic disease 16:4655-4665 PubMed39144301

2024

Differences in T cell-associated serum markers between ischemic cardiomyopathy and dilated cardiomyopathy.

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Yuli Huang,Lin Xuan,Qiong Xu,Jun Wang,Jie Liu

Scientific reports 14:11535 PubMed38773159

2024

Gamma radiation assisted green synthesis of hesperidin-reduced graphene oxide nanocomposite targeted JNK/SMAD4/MMP2 signaling pathway.

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Ahmad S Kodous,Eman O Taha,Dina F El-Maghraby,Asmaa A Hassana,M M Atta

Frontiers in cardiovascular medicine 11:1360364 PubMed38576426

2024

SARS-CoV-2 infection of endothelial cells, dependent on flow-induced ACE2 expression, drives hypercytokinemia in a vascularized microphysiological system.

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Christopher J Hatch,Sebastian D Piombo,Jennifer S Fang,Johannes S Gach,Makena L Ewald,William K Van Trigt,Brian G Coon,Jay M Tong,Donald N Forthal,Christopher C W Hughes

Heliyon 9:e17366 PubMed37426804

2023

circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.

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Unspecified application

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Lin Yang,Yuhao Lin,Chao Wang,Pengcheng Fan

Journal of applied toxicology : JAT 43:1613-1629 PubMed37278136

2023

Integrative proteomics and metabolomics analysis of non-observable acute effect level PM induced accumulative effects in AC16 cells.

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Bingru Nan,Xia Sun,Shijing Yang,Qingyu Huang,Heqing Shen

Redox biology 62:102681 PubMed37003179

2023

MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.

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Species

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Nunzia D'Onofrio,Francesco Prattichizzo,Elisa Martino,Camilla Anastasio,Luigi Mele,Rosalba La Grotta,Celestino Sardu,Antonio Ceriello,Raffaele Marfella,Giuseppe Paolisso,Maria Luisa Balestrieri

Journal of clinical laboratory analysis 36:e24685 PubMed36045604

2022

Serum VCAM-1 and ICAM-1 measurement assists for MACE risk estimation in ST-segment elevation myocardial infarction patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancai Yu,Yongxing Liu,Wanzhong Peng,Zesheng Xu

View all publications

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Human VCAM1 ELISA Kit