AB323603

Human UCHL1 ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human UCHL1 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human UCHL1 with a sensitivity of 7.621 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1

3 Images

Sandwich ELISA - Human UCHL1 ELISA Kit- Intracellular (AB323603)

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Intracellular (AB323603)

Example of human UCHL1 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Intracellular (AB323603)

Interpolated concentration of native UCHL1 was measured in duplicate in the following species samples : human brain cortex tissue extract, mouse brain tissue extract, and rat brain tissue extract. Undiluted samples are 500 ng/mL. The interpolated values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Intracellular (AB323603)

Interpolated concentration of native UCHL1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : brain cortex tissue extract 500 ng/mL, SH-SY5Y cell extract 500 ng/mL, and A549 cell extract 4 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Extracts, Cell Lysate

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 7.621 pg/mL

検出範囲

25 - 1600 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human UCHL1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of UCHL1 protein in Tissue extracts, Cell Lysate. Quantitate Human UCHL1 with 7.621 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips.

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.1" } ]

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回収率

[ { "sample": "Tissue Extracts", "range": "98 - 111 %", "average": "= 105" }, { "sample": "Cell Lysate", "range": "94 - 110 %", "average": "= 100" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323603-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323603-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human UCHL1 Detector Antibody", "number":"AB323603-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human UCHL1 Capture Antibody", "number":"AB323603-CMP01" }, { "size":"20 x 1 Vial", "name":"Human UCHL1 Lyophilized Recombinant Protein", "number":"AB323603-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323603-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323603-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323603-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323603-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323603-CMP04" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB323603-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323603-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323603-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human UCHL1 Detector Antibody", "number":"AB323603-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human UCHL1 Capture Antibody", "number":"AB323603-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human UCHL1 Lyophilized Recombinant Protein", "number":"AB323603-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323603-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323603-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323603-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323603-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB323603-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323603-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323603-CMP10", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed : 22212137, PubMed : 23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed : 22212137, PubMed : 25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed : 12408865, PubMed : 8639624, PubMed : 9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed : 22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed : 25615526).

See full target information UCHL1

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websiteProtocolBooklet

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