AB192149

Human TSLP ELISA Kit

SimpleStep
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Human TSLP ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human TSLP with a sensitivity of 3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326747

別名を表示する

Thymic stromal lymphopoietin, TSLP

5 Images

ELISA

Supplier Data

ELISA - Human TSLP ELISA Kit (AB192149)

sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Titration of normal Human serum (NHS) native and spiked with 0.5 ng/mL recombinant TSLP protein to within the working range of the assay.

The samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Assay for TSLP levels from 10 Individual Donor Human Serum samples and patients clinically diagnosed with Atopic Dermatitis (A.D.) to within the working range of the assay.

Severity of A.D. symptoms and medical histories of all donors are unknown. Samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Example of TSLP standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Example of TSLP standard curve prepared in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 3 pg/mL

検出範囲

15.6 - 1000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human TSLP ELISA Kit ab192149 is a rapid single-wash 90-min Sandwich ELISA to measure Human TSLP in cell culture supernatant, serum. This SimpleStep sensitivity is 3 pg/mL.

How the assay works

Human TSLP SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TSLP ELISA Kit ab192149 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Thymic stromal lyphopoietin (TSLP) is a protein belonging to the IL-7 cytokine family. TSLP is released by epithelial cells and keratinocytes in response to trauma, infection or allergen. It acts upon dendritic cells to mediate TH2 mediated immune response and appears to be a key mediator in many inflammatory diseases such as: Asthma, Eczema, and Atopic Dermatitis. TSLP also acts upon mast cells promote the release of IL-5, -6, and -13 in the presence of IL-1 and TNF-?.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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回収率

[ { "sample": "Serum", "range": "83 - 93 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "114 - 126 %", "average": "= 121" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7R?).

Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7R? receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.

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製品プロトコール

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ターゲットの情報

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.

See full target information TSLP

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 10:6585-6597 PubMed30746204

2019

Excessive periostin expression and Th2 response in patients with nasal polyps: association with asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wei,Renqiang Ma,Jia Zhang,Xingmei Wu,Guodong Yu,Xianting Hu,Jian Li,Zhuofu Liu,Wendong Ji,Huabin Li,Weiping Wen

View all publications

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websiteProtocolBooklet

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Human TSLP ELISA Kit