AB223594

Human TSC-1 ELISA Kit

Recombinant
SimpleStep
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Human TSC-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TSC-1 with a sensitivity of 1.54 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

SCYA26, UNQ216/PRO242, CCL26, C-C motif chemokine 26, CC chemokine IMAC, Eotaxin-3, Macrophage inflammatory protein 4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, MIP-4-alpha, TSC-1

3 Images

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

sELISA

Supplier Data

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Interpolated concentrations of native Eotaxin 3 in human A549 cell culture supernatant samples.

The concentrations of Eotaxin 3 were measured in duplicates, interpolated from the Eotaxin 3 standard curves and corrected for sample dilution. Undiluted human A549 supernatant : 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 5559.17 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Interpolated concentrations of spiked Eotaxin 3 in serum and plasma.

Interpolated concentrations of spiked Eotaxin 3 in human serum 50%, plasma (citrate) 50% and plasma (EDTA) 50%. The concentrations of Eotaxin 3 were measured in duplicate and interpolated from the Eotaxin 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 161 pg/mL in human serum and 320.02 pg/mL in citrate plasma, 318.05 pg/ml in EDTA plasma.

sELISA

Supplier Data

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Example of Human Eotaxin 3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 1.54 pg/mL

検出範囲

4.69 - 300 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human TSC-1 ELISA Kit (ab223594) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSC-1 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TSC-1 with 1.54 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

TSC-1 (Eotaxin 3 / CCL26) is a small cytokine within the CC chemokine family coded by a gene located on human chromosome 7. TSC-1 is also know by Macrophage inflammatory protein 4-alpha, Thymic stroma chemokine-1 and IMAC. It has known expression in heart, lung and ovarian tissue. TSC-1 expression can be induced by IL-4 stimulation which then can activate chemotaxic activity of eosinophils and basophiles by binding to the cell surface chemokine receptor CCR3.

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精度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "95 - 108 %", "average": "= 101" }, { "sample": "Serum", "range": "90 - 118 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "85 - 109 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "83 - 117 %", "average": "= 102" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tuberous Sclerosis Complex 1 (TSC-1) also known as hamartin is a protein with a molecular mass of approximately 130 kDa. It plays a direct role in cellular signaling and regulatory functions. TSC-1 is expressed broadly across tissues which suggests its importance in maintaining normal cellular physiology. It interfaces with various signaling molecules and directly interacts with TSC-2 to form a protein complex.

Biological function summary

TSC-1 plays a regulatory role in cell growth and proliferation. It forms a complex with TSC-2 also known as tuberin which is important for its function. This complex acts as a GAP (GTPase-activating protein) toward Rheb a small GTPase inhibiting the mTOR signaling pathway. The proper regulation of this pathway ensures controlled cell size and growth emphasizing the importance of the TSC-1/TSC-2 complex in cellular regulation.

Pathways

TSC-1 operates within the mTOR signaling pathway to influence cellular processes like autophagy and protein synthesis. It also relates to the PI3K/AKT pathway one that is involved in the regulation of cell survival and metabolism. By interacting with TSC-2 in these pathways TSC-1 modulates the inhibition of mTORC1 thereby impacting cellular homeostasis and growth.

Defects in TSC-1 can lead to tuberous sclerosis complex a condition characterized by benign tumors in multiple organs. Mutations in TSC-1 and its partner TSC-2 disrupt normal cellular functions leading to this disease. Additionally aberrations in the mTOR pathway influenced by TSC-1 malfunction have associations with certain cancers highlighting the importance of TSC-1 in maintaining cell proliferation and signaling balance.

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製品プロトコール

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ターゲットの情報

Chemoattractant for eosinophils and basophils (PubMed : 10415065, PubMed : 10488147). Acts as a ligand for C-C chemokine receptor CCR3 which triggers Ca(2+) mobilization in eosinophils (PubMed : 10415065, PubMed : 10488147, PubMed : 11425309). Also acts as a ligand for CX3C chemokine receptor CX3CR1, inducing cell chemotaxis (PubMed : 20974991).

See full target information CCL26

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websiteProtocolBooklet

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Human TSC-1 ELISA Kit