AB322439

Human TROP2 ELISA Kit- Extracellular

Recombinant
SimpleStep
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Human TROP2 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human TROP2 with a sensitivity of 1.623 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

5 Images

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Interpolated concentration of native TROP2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 6.25% A-431 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Interpolated concentration of native TROP2 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% A-431 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TROP2 concentration was determined to be 116.15 pg/mL with a range of 88.9 – 140.6 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Example of human TROP2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Example of human TROP2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 1.623 pg/mL

検出範囲

9.375 - 600 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Human TROP2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TROP2 protein in Serum, Cit Plasma, EDTA Plasma, and Cell culture supernatant. Quantitate Human TROP2 with 1.623 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

精度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

style

left-column

回収率

[ { "sample": "Serum", "range": "86 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "104 - 109 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "86 - 113 %", "average": "= 100" }, { "sample": "Cell culture supernatant", "range": "88 - 93 %", "average": "= 91" } ]

style

left-column

製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322439-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322439-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322439-CMP03" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322439-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB322439-CMP09" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human TROP2 Capture Antibody", "number":"AB322439-CMP01" }, { "size":"20 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB322439-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322439-CMP11" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322439-CMP06" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322439-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322439-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322439-CMP12" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322439-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322439-CMP04" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322439-CMP03" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322439-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB322439-CMP09" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human TROP2 Capture Antibody", "number":"AB322439-CMP01" }, { "size":"2 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB322439-CMP10" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322439-CMP12" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322439-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322439-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322439-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322439-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322439-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322439-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322439-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322439-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB322439-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human TROP2 Capture Antibody", "number":"AB322439-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB322439-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322439-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322439-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322439-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322439-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322439-CMP06", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

May function as a growth factor receptor.

See full target information TACSTD2

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com