Human Anti-Trichinella spiralis IgG ELISA Kit (ab108780)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Human Anti-Trichinella spiralis IgG ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Pos. Serum 8 3.9% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Pos. Serum 4 5.2% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
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アプリケーション
適用あり: ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Colored yellow; White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Trichinella spiralis (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Trichinella spiralis IgG Cut-off Control Colored yellow; Green cap 1 x 3ml Trichinella spiralis IgG Negative Control Colored yellow; Blue cap 1 x 2ml Trichinella spiralis IgG Positive Control Colored yellow; Red cap 1 x 2ml Trichinella spiralis Protein A HRP Conjugate Colored blue; Black cap 1 x 20ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108780の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
画像
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108780 は 1 報の論文で使用されています。
- Liu Y et al. Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera. PLoS Negl Trop Dis 15:e0009723 (2021). PubMed: 34432796