Human Transferrin ELISA Kit (ab187391)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.39 ng/ml
- Range: 0.63 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Transferrin ELISA Kit
Transferrin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 8 5.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 3 8.3% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.39 ng/ml -
検出範囲
0.63 ng/ml - 40 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 92 86% - 104% Cell culture media 101 97% - 103% Hep Plasma 114 112% - 116% EDTA Plasma 107 95% - 115% Cit plasma 115 104% - 121% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Transferrin ELISA Kit (ab187391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Transferrin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transferrin with 0.39 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human Transferrin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, and cell/tissue extract samples have not been tested with this kit.
CROSS REACTIVITY Human albumin, ferritin, and hemoglobin were prepared at 40 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
SPECIES REACTIVITY This kit recognizes human Transferrin protein.
Other species reactivity was determined by measuring 1:50,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution. Reactivity < 3% was determined for the following species: Mouse, Rat, Cow -
特記事項
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 384 tests 10X Human Transferrin Capture Antibody 1 x 600µl 1 x 600µl 10X Human Transferrin Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 2 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 1 x 6ml Human Transferrin Lyophilized Purified Protein (ab91435) 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 2 x 50ml 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
研究分野
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機能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
配列類似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
参照データベース
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
関連製品
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ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187391の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of human Transferrin standard curve in Sample Diluent NS in 96-well vs. 384-well plate
Example of human Transferrin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Transferrin in serum in 96-well vs. 384-well platesInterpolated concentration of native Transferrin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:50,000 human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Human Transferrin standard curve comparisonStandard Curve comparison between the original Human Transferrin SimpleStep ELISA kit and current Human Transferrin SimpleStep ELISA kit.
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Example of human Transferrin standard curve in Sample Diluent NS.The Transferrin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Transferrin standard curve in Sample Diluent NS. The Transferrin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Transferrin in human serum and plasmas samples.The concentrations of Transferrin were measured in duplicates, interpolated from the Transferrin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:50,000, plasma (EDTA) 1:50,000, plasma (citrate) 1:50,000 and plasma (heparin) 1:50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin concentration was determined to be 1.82 mg/mL in serum, 1.84 ng/mL in plasma (EDTA) and 1.86 mg/mL in plasma (citrate) and 1.43 ng/mL in plasma (heparin).
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Serum from seven individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin concentration was determined to be 2.38 mg/mL with a range of 1.54 – 3.2 mg/mL.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Transferrin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human Transferrin ELISA Kit (ab187391)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human Transferrin ELISA Kit (ab187391)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human Transferrin ELISA Kit (ab187391)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab187391 は 5 報の論文で使用されています。
- Hamdan Almaeen A & Mostafa-Hedeab G Haematological Indicators of Response to Erythropoietin Therapy in Chronic Renal Failure Patients on Haemodialysis: Impact of Angiotensin-Converting Enzyme rs4343 Gene Polymorphism. Pharmgenomics Pers Med 14:1055-1068 (2021). PubMed: 34483678
- Harder NHO et al. Effects of Dietary Glucose and Fructose on Copper, Iron, and Zinc Metabolism Parameters in Humans. Nutrients 12:N/A (2020). PubMed: 32854403
- Vatine GD et al. Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications. Cell Stem Cell 24:995-1005.e6 (2019). PubMed: 31173718
- Allden SJ et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med N/A:N/A (2019). PubMed: 31051082
- Anantpadma M et al. Large Scale Screening and Identification of Novel Ebolavirus and Marburgvirus Entry Inhibitors. Antimicrob Agents Chemother N/A:N/A (2016). Sandwich ELISA ; Human . PubMed: 27161622