Human Transferrin ELISA Kit (ab108911)
Key features and details
- Sensitivity: 89 ng/ml
- Range: 0.4 µg/ml - 6.25 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human Transferrin ELISA Kit
Transferrin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.1% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
= 89 ng/ml -
検出範囲
0.4 µg/ml - 6.25 µg/ml -
添加回収試験
96 %
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全工程の試験時間
2h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Transferrin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in plasma and serum samples.
A Transferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Transferrin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Transferrin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Competitive ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 6X Biotinylated Human Transferrin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Human Transferrin Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml Transferrin Microplate (12 x 8 well strips) 1 unit -
研究分野
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機能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
配列類似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
参照データベース
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108911の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108911
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab108911 は 15 報の論文で使用されています。
- Oldfield L et al. Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes. EBioMedicine 75:103802 (2022). PubMed: 34990893
- Hu Y et al. Clinical Characteristics, Iron Metabolism and Neuroinflammation: New Insight into Excessive Daytime Sleepiness in Parkinson's Disease. Neuropsychiatr Dis Treat 17:2041-2051 (2021). PubMed: 34188474
- Rodríguez-Mortera R et al. Higher Hepcidin Levels in Adolescents with Obesity Are Associated with Metabolic Syndrome Dyslipidemia and Visceral Fat. Antioxidants (Basel) 10:N/A (2021). PubMed: 34065056
- Mateos J et al. Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection. Sci Rep 10:3844 (2020). PubMed: 32123229
- Lian TH et al. Tremor-Dominant in Parkinson Disease: The Relevance to Iron Metabolism and Inflammation. Front Neurosci 13:255 (2019). PubMed: 30971879