Human TRAIL ELISA Kit (CD253) (ab46074)
Key features and details
- Sensitivity: 64 pg/ml
- Range: 93.75 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human TRAIL ELISA Kit (CD253)
TRAIL キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 40 1500pg/ml 82.9 5.5% B 33 116.3pg/ml 8.7 7.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A 12 1619pg/ml 62.8 3.8% B 12 162.8pg/ml 13.1 8% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 64 pg/ml -
検出範囲
93.75 pg/ml - 3000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human TRAIL (CD253) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab46074) is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.
A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.
This kit will recognize both endogenous and recombinant Human TRAIL.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-TRAIL Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml Control Silver 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml Plastic Plate Covers 4 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl TRAIL Microplate (12 x 8 well strips) 2 units 1 unit TRAIL Standard (Lyophilized) Yellow 4 vials 2 vials -
研究分野
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関連性
TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. -
細胞内局在
Cell Membrane; single-pass type II membrane protein. -
別名
- Apo 2 ligand
- APO2L
- CD253
see all -
参照データベース
- Entrez Gene: 8743 Human
- SwissProt: P50591 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46074の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)
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ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)TRAIL measured in undiluted biological fluids (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab46074
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab46074 は 6 報の論文で使用されています。
- King JL et al. Injectable pH Thermo-Responsive Hydrogel Scaffold for Tumoricidal Neural Stem Cell Therapy for Glioblastoma Multiforme. Pharmaceutics 14:N/A (2022). PubMed: 36297678
- Kitamura Y et al. Anti-EGFR VHH-armed death receptor ligand-engineered allogeneic stem cells have therapeutic efficacy in diverse brain metastatic breast cancers. Sci Adv 7:N/A (2021). PubMed: 33658202
- Read SA et al. Macrophage Coordination of the Interferon Lambda Immune Response. Front Immunol 10:2674 (2019). PubMed: 31798594
- Kurt IC et al. KDM2B, an H3K36-specific demethylase, regulates apoptotic response of GBM cells to TRAIL. Cell Death Dis 8:e2897 (2017). PubMed: 28661478
- Wang J et al. Silencing the epigenetic silencer KDM4A for TRAIL and DR5 simultaneous induction and antitumor therapy. Cell Death Differ 23:1886-1896 (2016). Human . PubMed: 27612013
- Greenwood EJ et al. Simian Immunodeficiency Virus Infection of Chimpanzees (Pan troglodytes) Shares Features of Both Pathogenic and Non-pathogenic Lentiviral Infections. PLoS Pathog 11:e1005146 (2015). Sandwich ELISA . PubMed: 26360709