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Human Anti-Toxocara canis IgG ELISA Kit (ab108775)

Submit a review Q&A (1)References (1)
Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Anti-Toxocara canis IgG ELISA Kit

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Pos Serum 12 2.6%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Pos. Serum 5 8.1%

    • サンプルの種類

      Serum, Hep Plasma, Cit plasma

    • アッセイタイプ

      Indirect

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s anti-Toxocara canis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Toxocara canis in Human serum and plasma.


      A 96-well plate has been precoated with Toxocara canis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Toxocara canis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxocara canis IgG sample captured in plate.

    • アプリケーション

      適用あり: ELISAmore details

    • 試験プラットフォーム

      Microplate

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab108775の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    Use at an assay dependent concentration.
    特記事項
    ELISA
    Use at an assay dependent concentration.

    画像

    • Serologic ELISA assay principle
      Serologic ELISA assay principle
      Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

    参考文献 (1)

    ab108775 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108775 は 1 報の論文で使用されています。

    • Phasuk N & Punsawad C Seroprevalence of Toxocara canis infection and associated risk factors among primary schoolchildren in rural Southern Thailand. Trop Med Health 48:23 (2020). PubMed: 32336929

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Dear Sirs,
    for one of our customers we are searching for special tests for Toxocara
    canis etc.
    Could you please send us an updated offer for:
    ab108775
    Toxocara canis IgG Human ELISA Kit (ab108775)
    Additionally if you have a similar test for nematoda in cats important
    to test in Humans this would be of interest as well.
    We would need the pricing information + shipping costs, availablity as
    well as the data sheet please. Also exp. dates would be of interest!
    If there are additional consumables needed please let us know as well.
    We would be happy to support our customer with your products.
    Many thanks in advance,

    Read More
    Answer

    Thank you for your inquiry.

    I just send an quotation in a separate email with all shipping costs, etc.

    We do not have set expiry dates at Abcam, since antibodies and most of our otherproducts can last very long if stored properly. I can confirm that the reagents are stable up to the expiry date stated on the label when stored at 2-8 °C.

    We do have our Abcam guarantee that covers all our products for six month.

    To find out more please follow this link:

    https://www.abcam.com/abpromise

    Please follow this link to the datasheet which has also has a link tothe protocol booklet at the bottom:

    https://www.abcam.com/Human-Toxocara-canis-IgG-ELISA-Kit-ab108775.html (or use the following: https://www.abcam.com/Human-Toxocara-canis-IgG-ELISA-Kit-ab108775.html).

    The datasheet and protocol booklet will be send in printedform with the kit too.

    Unfortunately, we do not offer a nematode from cat (human IgG ELISA) kit. I will be happy to double check if you are looking for specific nematodes?

    Please let me know if you have any further questions.

    Read More

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