Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントSimpleStep

Human TNSF18/GITRL ELISA Kit (ab300314)

Submit a review Submit a question
Example of human TNSF18/GITRL standard curve in Sample Diluent NS.
  • Interpolated concentrations of spiked human TNSF18/GITRL in serum, plasma-citrate, plasma-edta, plasma-heparin, and cell culture supernatant.
  • Interpolated concentrations of TNSF18/GITRL in normal human serum donors.
  • Human TNSF18/GITRL ELISA Kit (ab300314)
  • Human TNSF18/GITRL ELISA Kit (ab300314)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.276 pg/ml
  • Range: 12.5 pg/ml - 800 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human TNSF18/GITRL ELISA Kit
    TNFSF18/GITRL キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Serum 8 4.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Serum 3 5%

  • サンプルの種類

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    3.276 pg/ml

  • 検出範囲

    12.5 pg/ml - 800 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 93 84% - 98%
    Serum 102 95% - 111%
    Hep Plasma 96 88% - 101%
    EDTA Plasma 91 85% - 100%
    Cit plasma 96 88% - 102%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human TNSF18/GITRL SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNSF18/GITRL protein in human serum, plasma and cell culture supernatant. Quantitate Human TNSF18/GITRL with 3.276 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 96 tests
    10X Human TNSF18/GITRL Capture Antibody 1 x 600µl
    10X Human TNSF18/GITRL Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human TNSF18/GITRL Lyophilized Recombinant Protein 2 vials
    Plate Seal 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.

  • 組織特異性

    Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 別名

    • Activation inducible TNF related ligand
    • Activation-inducible TNF-related ligand
    • AITR ligand
    • AITRL
    • GITR ligand
    • GITRL
    • Glucocorticoid induced TNF related ligand
    • Glucocorticoid induced TNFR related protein ligand
    • Glucocorticoid-induced TNF-related ligand
    • hGITRL
    • MGC138237
    • TL6
    • TNF18_HUMAN
    • TNFSF18
    • TNLG2A
    • Tumor necrosis factor (ligand) superfamily member 18
    • Tumor necrosis factor ligand 2A
    • Tumor necrosis factor ligand superfamily member 18
    see all

  • 参照データベース

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab300314の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Example of human TNSF18/GITRL standard curve in Sample Diluent NS.
      Example of human TNSF18/GITRL standard curve in Sample Diluent NS.

      Example of human TNSF18/GITRL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked human TNSF18/GITRL in serum, plasma-citrate, plasma-edta, plasma-heparin, and cell culture supernatant.
      Interpolated concentrations of spiked human TNSF18/GITRL in serum, plasma-citrate, plasma-edta, plasma-heparin, and cell culture supernatant.

      Recombinant TNSF18/GITRL was spiked into the following human serum, plasma, and cell culture supernatant samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% serum, 50% plasma-citrate, 50% plasma-EDTA, 50% plasma-heparin, and 50% cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Interpolated concentrations of TNSF18/GITRL in normal human serum donors.
      Interpolated concentrations of TNSF18/GITRL in normal human serum donors.

      Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean TNSF18/GITRL concentration of detected samples was determined to be 21.7 pg/mL with a range of undetected – 38.2 pg/mL.

    • Human TNSF18/GITRL ELISA Kit (ab300314)
      Human TNSF18/GITRL ELISA Kit (ab300314)
    • Human TNSF18/GITRL ELISA Kit (ab300314)
      Human TNSF18/GITRL ELISA Kit (ab300314)

    参考文献 (0)

    ab300314 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300314 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab300314.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com