Human TNFSF18 / GITRL ELISA Kit (ab113323)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 41 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human TNFSF18 / GITRL ELISA Kit
TNFSF18/GITRL キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 25 pg/ml -
検出範囲
41 pg/ml - 10000 pg/ml -
添加回収試験
97 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 87.83 67% - 100% Serum 116.9 108% - 123% Plasma 87.69 67% - 100% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human TNFSF18 / GITRL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFSF18 / GITRL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human TNFSF18 / GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFSF18 / GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFSF18 / GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFSF18 / GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human GITR Ligand Item F 2 vials TNFSF18 Ligand Microplate (12 x 8 wells) 1 unit Recombinant Human GITR Ligand Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. -
組織特異性
Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Membrane. - Information by UniProt
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別名
- Activation inducible TNF related ligand
- Activation-inducible TNF-related ligand
- AITR ligand
see all -
参照データベース
- Entrez Gene: 8995 Human
- Omim: 603898 Human
- SwissProt: Q9UNG2 Human
- Unigene: 248197 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical data
Representative standard curve using ab113323.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113323 は論文での使用が確認できていません。