Human Tissue Type Plasminogen Activator ELISA Kit (TPA) (ab108914)
Key features and details
- Sensitivity: 4.7 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human Tissue Type Plasminogen Activator ELISA Kit (TPA)
Tissue Plasminogen Activator キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
>= 4.7 pg/ml -
検出範囲
15.625 pg/ml - 1000 pg/ml -
添加回収試験
98 %
-
全工程の試験時間
4h 0m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Tissue Type Plasminogen Activator (TPA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Type Plasminogen Activator in plasma, serum, urine, saliva, milk, cell culture supernatants and CSF samples.
A TPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TPA captured in plate.
Get results in 90 minutes with Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 50X Biotinylated Human TPA Antibody 1 x 120µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml TPA Microplate (12 x 8 well strips) 1 unit TPA Standard 1 vial -
研究分野
-
機能
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
組織特異性
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
関連疾患
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
ドメイン
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
翻訳後修飾
The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
-
別名
- Alteplase
- DKFZp686I03148
- Plasminogen activator tissue
see all -
参照データベース
- Entrez Gene: 5327 Human
- Omim: 173370 Human
- SwissProt: P00750 Human
- Unigene: 491582 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108914の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
-
Human Tissue Type Plasminogen Activator ELISA Kit (TPA)
Representative Standard Curve Using ab108914.
-
TPA - Human ELISA Kit (ab108914)Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 2-10 fold.
-
TPA - Human ELISA Kit (ab108914)Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Human samples diluted 3-27 fold. Rat and mouse samples diluted 1-10 fold.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab108914 は 8 報の論文で使用されています。
- Ide R et al. Comparative analysis of hyperfibrinolysis with activated coagulation between amniotic fluid embolism and severe placental abruption. Sci Rep 14:272 (2024). PubMed: 38168649
- Wang S et al. LncRNA NEAT1 Promotes Vascular Endothelial Cell Dysfunction via miR-218-5p/GAB2 and Serves as a Diagnostic Biomarker for Deep Vein Thrombosis. Clin Appl Thromb Hemost 29:10760296231179447 (2023). PubMed: 37321605
- Pentz R et al. Nerve growth factor (NGF) pathway biomarkers in Down syndrome prior to and after the onset of clinical Alzheimer's disease: A paired CSF and plasma study. Alzheimers Dement 17:605-617 (2021). PubMed: 33226181
- Monteiro LJ et al. Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy. Diagnostics (Basel) 10:N/A (2020). PubMed: 32244842
- Takeishi K et al. Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells. Cell Rep 31:107711 (2020). PubMed: 32492423
- Jia H et al. Endothelial cell functions impaired by interferon in vitro: Insights into the molecular mechanism of thrombotic microangiopathy associated with interferon therapy. Thromb Res 163:105-116 (2018). PubMed: 29407621
- Faine BA et al. Helicopter "Drip and Ship" Flights Do Not Alter the Pharmacological Integrity of rtPA. J Stroke Cerebrovasc Dis 27:2720-2724 (2018). PubMed: 30037651
- Chung HJ et al. Production of human tissue-type plasminogen activator (htPA) using in vitro cultured transgenic pig mammary gland cells. Anim Biotechnol N/A:1-6 (2018). PubMed: 30522372