Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904)
Key features and details
- Sensitivity: 0.081 ng/ml
- Range: 0.625 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI)
TFPI キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.9% -
サンプルの種類
Cell culture supernatant, Milk, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
> 0.081 ng/ml -
検出範囲
0.625 ng/ml - 5 ng/ml -
添加回収試験
98 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.
A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.
Get results in 90 minutes with Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Tissue Factor Pathway Inhibitor Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Tissue Factor Pathway Inhibitor Microplate (12 x 8 well strips) 1 x 96 units Tissue Factor Pathway Inhibitor Standard 2 vials -
研究分野
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機能
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
組織特異性
Mostly in endothelial cells. -
配列類似性
Contains 3 BPTI/Kunitz inhibitor domains. -
ドメイン
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Anti convertin
- EPI
- Extrinsic pathway inhibitor
see all -
参照データベース
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108904の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - ab108904 Tissue Factor Pathway Inhibitor (TFPI) Human ELISA Kit
TFPI measured in various samples showing quantity (ng) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108904.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab108904 は 6 報の論文で使用されています。
- Ma XP et al. Transthyretin increases migration and invasion of rat placental trophoblast cells. FEBS Open Bio 10:1568-1576 (2020). PubMed: 32533762
- Martínez González J et al. Bioequivalence of a biosimilar enoxaparin sodium to Clexane® after single 100 mg subcutaneous dose: results of a randomized, double-blind, crossover study in healthy volunteers. Drug Des Devel Ther 12:575-582 (2018). PubMed: 29593380
- Ansell JE et al. Single-dose ciraparantag safely and completely reverses anticoagulant effects of edoxaban. Thromb Haemost 117:238-245 (2017). PubMed: 27853809
- Vieira ML et al. Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. PLoS Negl Trop Dis 10:e0004713 (2016). PubMed: 27167223
- Abu El-Asrar AM et al. Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy. Mol Vis 22:424-35 (2016). PubMed: 27168718
- Park MY et al. Biomarkers of kidney injury and klotho in patients with atherosclerotic renovascular disease. Clin J Am Soc Nephrol 10:443-51 (2015). Sandwich ELISA ; Human . PubMed: 25542906