Human TIMP1 ELISA Kit (ab187394)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human TIMP1 ELISA Kit
TIMP1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 4.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 0.6% -
サンプルの種類
Cell culture supernatant, Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
17 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 89 86% - 92% Saliva 84 82% - 87% Serum 89 89% - 90% Hep Plasma 95 94% - 98% EDTA Plasma 89 86% - 91% Cit plasma 97 95% - 99% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human TIMP1 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human TIMP1 ELISA Kit (ab187394) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP1 protein in cit plasma, edta plasma, hep plasma, serum, saliva, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TIMP1 with 17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Tissue inhibitor of metalloproteinases 1 (TIMP1 or Metalloproteinase inhibitor 1) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding non-covalently to MMPs (in a 1:1 stoichiometry) and blocking access of substrate to the MMP active site. TIMP1 expression is induced by pro-inflammatory cytokines. It is a soluble factor found circulating in serum and plasma. TIMP1 appears also have functions that are independent of MMP inhibition, including promoting erythropoiesis and inhibiting apoptosis in B cells.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Human TIMP-1 Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Human TIMP-1 Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 10 x 6ml 1 x 6ml Human TIMP-1 Lyophilized Recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seal 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究分野
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
参照データベース
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
関連製品
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Alternative Versions
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ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187394の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated concentrations of human TIMP-1 in serum in 96-well vs. 384-well plates.Interpolated concentration of native TIMP-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human TIMP-1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human TIMP-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Human TIMP-1 competitor curve comparisonStandard Curve comparison between Human TIMP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human TIMP-1 standard curve comparisonStandard Curve comparison between the original Human TIMP-1 SimpleStep ELISA kit and current Human TIMP-1 SimpleStep ELISA kit.
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Example of human TIMP-1 standard curve in Sample Diluent NS.The TIMP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TIMP-1 in human serum, and plasma samples.The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 95.9 ng/mL in serum, 74 ng/mL in plasma (citrate), 68 ng/mL in plasma (EDTA) and 56 ng/mL in plasma (heparin).
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 92.6 ng/mL with a range of 66.5 – 128.7 ng/mL.
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Interpolated concentrations of native TIMP-1 in human cell culture supernatant samples.The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC cell culture supernatant 20% and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 5 ng/mL in unstimulated and 22 ng/mL in stimulated and undetectable in media.
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Interpolated concentrations of native TIMP-1 in human saliva sample.The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 179.4 ng/mL in saliva.
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Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab187394 は 6 報の論文で使用されています。
- Qi Z et al. Effects of Surgery Combined with Different Chemotherapy on Matrix Metalloproteinase-9 and Tissue Inhibitors of Metalloproteinase-1 in Children with Neuroblastoma. J Oncol 2022:8319221 (2022). PubMed: 35847358
- Kirketerp-Møller K et al. Corrigendum: Biomarkers of Skin Graft Healing in Venous Leg Ulcers. Acta Derm Venereol 102:adv00771 (2022). PubMed: 36039412
- Capistrano da Silva E et al. Determining MMP-2 and MMP-9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer. Vet Ophthalmol 24:279-287 (2021). PubMed: 33834598
- Fragão-Marques M et al. Atrial matrix remodeling in atrial fibrillation patients with aortic stenosis. BMC Cardiovasc Disord 20:468 (2020). PubMed: 33129260
- Fuchs PÖ et al. Fibrin fragment E potentiates TGF-ß-induced myofibroblast activation and recruitment. Cell Signal 72:109661 (2020). PubMed: 32334027
- He Z et al. Cardiomyocyte-specific expression of CYP2J2 prevents development of cardiac remodelling induced by angiotensin II. Cardiovasc Res 105:304-17 (2015). PubMed: 25618409