AB314599

Human TIGIT ELISA Kit - Extracellular

SimpleStep
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Human TIGIT ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human TIGIT with a sensitivity of 7.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

別名を表示する

VSIG9, VSTM3, TIGIT, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

3 Images

Sandwich ELISA - Human TIGIT ELISA Kit - Extracellular (AB314599)

sELISA

Supplier Data

Sandwich ELISA - Human TIGIT ELISA Kit - Extracellular (AB314599)

Interpolated concentrations of TIGIT in normal Human serum donors

Serum of ten individual healthy Human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIGIT concentration was determined to be 43.8 pg/mL with a range of 14.4 – 83.4 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TIGIT ELISA Kit - Extracellular (AB314599)

Interpolated concentrations of spiked Human TIGIT in human serum, plasma (citrate), plasma (EDTA), plasma (heparin)

Recombinant TIGIT was spiked into the following human samples and diluted in a 2-fold dilution series in Sample diluent NS. Undiluted samples are 50% for serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human TIGIT ELISA Kit - Extracellular (AB314599)

Example of Human TIGIT standard curve in Sample diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 7.5 pg/mL

検出範囲

15.625 - 1000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human TIGIT SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIGIT protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma. Quantitate Human TIGIT with 7.5 pg/ml sensitivity. SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" } ]

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回収率

[ { "sample": "Serum", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "93 - 101 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "94 - 102 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "97 - 104 %", "average": "= 100" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.

Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.

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製品プロトコール

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ターゲットの情報

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed : 19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed : 23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed : 24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed : 32503945).

See full target information TIGIT

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websiteProtocolBooklet

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Abcam product promise

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Human TIGIT ELISA Kit - Extracellular