AB223595

Human Thymidine Kinase 1 ELISA Kit

Recombinant
SimpleStep
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Human Thymidine Kinase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thymidine Kinase 1 with a sensitivity of 6.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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ELISA - Human Thymidine Kinase 1 ELISA Kit (AB223595)

ELISA

Supplier Data

ELISA - Human Thymidine Kinase 1 ELISA Kit (AB223595)

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine Kinase 1 ELISA Kit (AB223595)

Example of human Thymidine Kinase 1 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine Kinase 1 ELISA Kit (AB223595)

Interpolated concentrations of native Thymidine Kinase 1 in human PC3, MCF7, and SH-SY5Y cell extracts.

Interpolated concentrations of native Thymidine Kinase 1 in human PC-3, MCF7, and SH-SY5Y cell extracts based on a 16 μg/mL extract load. The concentrations of Thymidine Kinase 1 were measured in duplicate and interpolated from the Thymidine Kinase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thymidine Kinase 1 concentration was determined to be 1251 pg/ml in PC-3, 585 pg/mL in MCF7, 721 pg/mL in SH-SY5Y.

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine Kinase 1 ELISA Kit (AB223595)

Interpolated concentrations of spiked Thymidine Kinase 1 in human serum, plasma and cell culture supernatant samples.

The concentrations of Thymidine Kinase 1 were measured in duplicates, interpolated from the Thymidine Kinase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 25% and HUVEC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine Kinase 1 ELISA Kit (AB223595)

Example of human Thymidine Kinase 1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 6.1 pg/mL

検出範囲

23.5 - 3500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Thymidine Kinase 1 ELISA Kit ab223595 is a rapid single-wash 90-min Sandwich ELISA to measure Human Thymidine Kinase 1 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 6.1 pg/mL.

How the assay works

Human Thymidine Kinase 1 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Thymidine Kinase 1 ELISA Kit ab223595 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Thymidine Kinase 1/TK1 antibody [EPR20203-90] - BSA and Azide free (Capture) ab244926
- Anti-Thymidine Kinase 1/TK1 antibody [EPR20203-203] - BSA and Azide free (Detector) ab244926

Thymidine kinase 1, or TK1, is a 234- amino acid, zinc binding protein encoded by the Thymidine Kinase 1 gene. The primary function of Thymidine Kinase 1 is the phosphorylation of thymidine to form deoxythymidine 5'-phosphate, dTMP, and is necessary for DNA replication. The activity of Thymidine Kinase 1 is highest during the S phase of cells, and lowers during the G2 phase. Two forms of Thymidine Kinase 1 have been identified, one in the cytosol and the other in mitochondria, which is known as TK2. The presence of Thymidine Kinase 1 in serum may be a biomarker for certain types of cancer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "95 - 104 %", "average": "= 100" }, { "sample": "Serum", "range": "75 - 91 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "80 - 81 %", "average": "= 80" }, { "sample": "Cell culture extracts", "range": "86 - 120 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "75 - 82 %", "average": "= 80" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine Kinase 1 (TK1) also known as Thymidine Kinase isoform A is an enzyme involved in the phosphorylation of thymidine into thymidine monophosphate an important component in DNA synthesis. TK1 has a molecular weight of approximately 25 kDa. It is predominantly expressed in proliferating cells with expression levels peaking during the S phase of the cell cycle. You often find TK1 in tissues with high cell division rates like bone marrow and lymphatic tissues.

Biological function summary

This enzyme is critical for DNA replication and repair by providing deoxythymidine triphosphate (dTTP) a deoxynucleoside triphosphate. TK1 operates as a monomer but can form a tetrameric complex in its active state which enhances its affinity for substrates. TK1 can process various substrates including thymidine and thymidine analogs such as 5-bromo-2'-deoxyuridine (BrdU) and EdU. These substrates are vital in molecular biology as they help in measuring DNA synthesis in cell proliferation studies.

Pathways

The function of TK1 is central to the salvage pathway of nucleotide synthesis. This pathway allows recycling of thymidine from degraded DNA which is particularly important for rapidly dividing cells that need to ensure a sufficient supply of nucleotides. TK1 acts alongside other enzymes such as thymidylate synthase and ribonucleotide reductase to maintain nucleotide balance within the cell ensuring proper DNA synthesis and cellular proliferation.

TK1 levels often increase in cancerous tissues due to their elevated cell proliferation rates. Serum TK1 is a biomarker for various cancers like leukemia and breast cancer. Studies show a correlation between high TK1 activity and tumor aggressiveness and progression. Additionally interactions of TK1 with the protein p53 a tumor suppressor show that defects in TK1 regulation can contribute to genomic instability highlighting its role in oncogenesis.

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製品プロトコール

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ターゲットの情報

Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed : 9575153). Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed : 22385435). Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed : 22385435).

See full target information TK1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

BioTechniques 68:334-341 PubMed32336110

2020

AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays.

Applications

Unspecified application

Species

Unspecified reactive species

Jagarlamudi Kiran Kumar,Sofia Holmgren,Kerstin Hamberg Levedahl,Martin Höglund,Per Venge,Staffan Eriksson

Frontiers in oncology 9:1475 PubMed32064235

2020

Knockdown of Thymidine Kinase 1 Suppresses Cell Proliferation, Invasion, Migration, and Epithelial-Mesenchymal Transition in Thyroid Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Jian Wang,Li Zhao,Hui He,Pan Zhao,Zheng Peng,Feiyuan Liu,Juan Chen,Weiqing Wu,Guangsuo Wang,Fajin Dong

View all publications

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websiteProtocolBooklet

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Human Thymidine Kinase 1 ELISA Kit