Human Thrombomodulin ELISA Kit (CD141) (ab46508)

Thank you very much for your understanding. The things are more clearer now. Lab has confirmed that the number K100624 (typos K10624) is indeed stands for standard vial.

They have also confirmed that the standard conc. should be between 11-16 ng/ml at 1ml reconstitution with diluent. The expected range value 11-16 ng/ml has been determined by 2 operators, 3 experiments, 6 replicates each.

So at this moment I am unsure about 7ng/ml reading of standard, which is about ˜50% lower than the expected. To understand this I have few questions to ask;

- Does all the 5 kits show conc. range of 7ng/ml?
- I have checked the order history of this kit and found that the same item was bought many times. Would you be so kind, letting me know the lot numbers affected by this problem. You have received >5 kits of batch number http://it.adminsite.com/admin/stock/stock_list.cfm?intAbID=46508&intStockID=1119463&fMain=1&fViewExpired=1&strViewOffice=%25 was this batch fine?
- Do you follow the protocol exactly as given on the datasheet? Have you changed any step?
- From you r experience do you think, only the standard is problematic? If you think other can also be, then which one you would choose?

I will look forward to hearing from you soon.

Thank you for your inquiry.


A panel of 40 human sera and 40 plasma samples were tested for CD141 in a recent batch. See results below

Sample Matrix Number of samples evaluated Range (ng/ml) Mean (ng/ml) Standard deviation (ng/ml)


Serum 40 1.9 – 11.2 7.1 2.5
Plasma 40 2.9 – 7.6 5.1 1.5


I hope this information helps. Please contact us with any other questions.

Thank you for your telephone enquiry earlier this afternoon. I can confirm that the concentration of the streptavidin - HRP in the Human Thrombomodulin ELISA Kit is 1.0mg/ml. I hope this is helpful. If you have any further questions, please do not hesitate to contact us again.

Thanks for your enquiry.

My colleague is out of office and he has asked me to look after her customers during her absence.


The whole Thrombomodulin kit is stable if refrigerate (4°C) until at least for one year. However, we would suggest our customers to use it as soon as possible after purchase to make sure that our Abpromise guarantee applies (6 months after purchase).

The cytokine provided (Thrombomodulin lyophilized standard vial) is stable at 4°C several years. When the material is reconstituted, the stability is not guarantee even if frozen at -70°C. The vial should be resuspended immediately prior to use and then discard.

I hope this will be useful for you.