Human TGF beta 1 ELISA Kit (ab108912)
Key features and details
- Sensitivity: 31 pg/ml
- Range: 0.063 ng/ml - 4 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human TGF beta 1 ELISA Kit
TGF beta 1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 60 6.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 60 10.9% -
サンプルの種類
Cell culture supernatant -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 31 pg/ml -
検出範囲
0.063 ng/ml - 4 ng/ml -
添加回収試験
108 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s TGF beta 1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TGF beta 1 concentrations in cell culture supernatants.
A TGF beta 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TGF beta 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TGF beta 1 captured in plate. This assay recognizes both natural and recombinant Human TGF beta 1.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 30X Biotinylated Human TGF beta 1 Antibody 1 x 180µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml TGF beta 1 Microplate (12 x 8 well strips) 1 unit TGF beta 1 Standard 1 vial -
研究分野
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機能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
組織特異性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
関連疾患
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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別名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
参照データベース
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108912.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab108912 は 8 報の論文で使用されています。
- Radstake WE et al. The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the In Vitro Wound Healing Process. Cells 12:N/A (2023). PubMed: 36672184
- Huang S et al. Melatonin influences the biological characteristics of keloid fibroblasts through the Erk and Smad signalling pathways. Burns Trauma 11:tkad005 (2023). PubMed: 36873285
- Zhao HC et al. CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis. iScience 26:106862 (2023). PubMed: 37275516
- Radstake WE et al. Gravitational effects on fibroblasts' function in relation to wound healing. NPJ Microgravity 9:48 (2023). PubMed: 37344509
- Lee CH et al. Isoliquiritigenin Inhibits Gastric Cancer Stemness, Modulates Tumor Microenvironment, and Suppresses Tumor Growth through Glucose-Regulated Protein 78 Downregulation. Biomedicines 10:N/A (2022). PubMed: 35740372
- Zhao Q et al. PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. Mol Cell 77:95-107.e5 (2020). PubMed: 31628042
- Li W et al. LncRNA POU3F3 promotes cancer cell migration and invasion in nasopharyngeal carcinoma by up-regulating TGF-ß1. Biosci Rep 39:N/A (2019). PubMed: 30602452
- Zhang H et al. lncRNA MIR4435-2HG promotes cancer cell migration and invasion in prostate carcinoma by upregulating TGF-ß1. Oncol Lett 18:4016-4021 (2019). PubMed: 31516603