AB285288

Human TERT ELISA Kit

Name: Human TERT ELISA Kit (ab285288) | Abcam
Brand: Abcam Limited
SKU: AB285288
Price: 147000 JPY
Availability: InStock

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Human TERT ELISA Kit is a Sandwich ELISA for the measurement of Human TERT in in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

別名を表示する

EST2, TCS1, TRT, TERT, Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2

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ELISA

Supplier Data

ELISA - Human TERT ELISA Kit (AB285288)

Human TERT ELISA Kit.

Standard curve using Human TERT ELISA Kit ab285288 where R² = 0.9888. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

アッセイタイプ

Sandwich

Quantitative

検出感度

< 0.094 ng/mL

検出範囲

0.156 - 10 ng/mL

アッセイプラットフォーム

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

This Telomerase reverse transcriptase (TERT) ELISA kit (ab285288, K4187) is a sandwich ELISA assay for the quantitative measurement of human TERT in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of TERT captured from the samples.

This product is manufactured by BioVision, an Abcam company and was previously called K4187 TERT (Human) ELISA Kit. K4187-100 is the same size as the 96 test size of ab285288.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Telomerase reverse transcriptase also known as TERT is an enzyme with a molecular mass of about 127 kDa. It functions as a catalytic subunit of the telomerase complex which extends telomeres by adding the nucleotide sequence "TTAGGG" to the ends of chromosomes. TERT operates with a telomerase RNA component (TERC) to synthesize DNA using an RNA template a process called reverse transcription. TERT is expressed in embryonic tissues adult stem cells and cancer cells where rapid cell division occurs.

Biological function summary

TERT plays an important role in maintaining telomere length essential for protecting chromosome stability. The protein forms part of the telomerase holoenzyme complex working together with TERC to perform its function. Telomerase activity allows cells to bypass the Hayflick limit preventing senescence and enhancing replication potential. In addition to telomere maintenance TERT influences gene regulation and cellular proliferation.

Pathways

TERT holds significance in the telomere maintenance and signaling pathways. Telomere maintenance ensures genomic integrity and cellular longevity a process fundamental for tissue homeostasis and regeneration. TERT interacts with proteins such as dyskerin and TERC within these pathways. Its regulatory roles in these pathways also reveal interactions with other key proteins like hypothesized targets in the DNA damage response.

The upregulation of TERT is associated with cancer and degenerative diseases. Increased telomerase activity is a common feature in many cancers leading to uncontrolled cell proliferation. Additionally mutations in TERT and its associated components can contribute to disorders like dyskeratosis congenita a rare genetic condition resulting in bone marrow failure. In these conditions TERT interaction with proteins involved in chromosomal maintenance and repair becomes important for understanding disease mechanisms.

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製品プロトコール

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ターゲットの情報

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

See full target information TERT

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Human TERT ELISA Kit