AB289696

Human TARC ELISA Kit

Recombinant
SimpleStep
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Human TARC ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human TARC with a sensitivity of 1.049 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab325909

別名を表示する

SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine

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Sandwich ELISA - Human TARC ELISA Kit (AB289696)

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Interpolated concentrations of TARC in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean TARC concentration of detected samples was determined to be 7.28 pg/mL with a range of undetected – 12.6 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Interpolated concentrations of human TARC in cell culture supernatant.

Interpolated concentration of native TARC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 20 PBMC treated with 1.5% PHA-M for 46 hours and 1 : 20 PBMC untreated. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Interpolated concentrations of human TARC in serum, plasma - citrate, and plasma - EDTA.

Interpolated concentration of native TARC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum neat, plasma - citrate 50%, and plasma - EDTA 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Human TARC competitor standard curve comparison.

Standard curve comparison between Human TARC SimpleStep ELISA kit (ab289696) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Human TARC standard curve comparison.

Standard curve comparison between the original Human TARC SimpleStep ELISA (ab183366) and current Human TARC SimpleStep ELISA (ab289696).

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Example of human TARC standard curve in Sample Diluent NS.

Example of human TARC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Interpolated concentration of native TARC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% stimulated PBMC supernatant (PHA-M, 46h). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human TARC ELISA Kit (AB289696)

Example of human TARC standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 1.049 pg/mL

検出範囲

3.906 - 250 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human TARC ELISA Kit (ab289696) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TARC in serum, plasma (EDTA), plasma (citrate), cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TARC with 1.049 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "89 - 95 %", "average": "= 93" }, { "sample": "Serum", "range": "85 - 91 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "91 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "87 - 91 %", "average": "= 89" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TARC also known as Thymus and Activation-Regulated Chemokine or CCL17 is a chemokine with a molecular mass of approximately 8 kDa. TARC is expressed primarily in the thymus and at lower levels in organs like the lung spleen and colon. It functions mechanically as a signaling molecule by binding to its receptor CCR4. This interaction drives the migration and activation of certain immune cells such as T helper 2 (Th2) cells which plays a role in immune response modulation.

Biological function summary

TARC is significant in the immune system's regulation. It is not part of a larger complex but operates independently to direct the movement of immune cells toward sites of inflammation or tissue damage. This chemokine attracts Th2 cells and can influence the balance of T cell responses important for maintaining immune homeostasis and responding to external pathogens. TARC also contributes to the maturation and activation of immune cells having an influential role in inflammatory processes.

Pathways

The TARC-CCR4 axis is integral to the immune response pathway and the inflammatory signaling pathway. Through these pathways TARC interacts with other proteins like CCL22 which also binds to CCR4 working systematically to guide cell movement and modulate immune functions. It ensures that immune responses are directed accurately which is central within different signaling cascades.

TARC is highly relevant to conditions such as atopic dermatitis and asthma. These diseases involve improper immune responses where TARC's role in attracting Th2 cells contributes to the inflammatory environment. In atopic dermatitis TARC levels are often elevated indicating its strong link to disease progression. Connections also exist between TARC and other proteins like IL-4 and IL-13 which are implicated in asthma facilitating a further understanding of the pathogenesis of these conditions.

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製品プロトコール

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ターゲットの情報

Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed : 8702936, PubMed : 9169480). Acts by binding to CCR4 at T-cell surface (PubMed : 10540332, PubMed : 9169480). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed : 27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (By similarity). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (By similarity).

See full target information CCL17

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websiteProtocolBooklet

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Human TARC ELISA Kit