AB326741

Human SynCAM/CADM1 ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
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Human SynCAM/CADM1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human SynCAM/CADM1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 9.14 - 20000 pg/ml with a sensitivity of 5.72 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

別名を表示する

IGSF4, IGSF4A, NECL2, SYNCAM, TSLC1, CADM1, Cell adhesion molecule 1, Immunoglobulin superfamily member 4, Nectin-like protein 2, Spermatogenic immunoglobulin superfamily, Synaptic cell adhesion molecule, Tumor suppressor in lung cancer 1, IgSF4, NECL-2, SgIgSF, SynCAM, TSLC-1

4 Images

Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)

sELISA

Supplier Data

Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)

Serum of ten individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean SynCAM/CADM1 concentration was determined to be 3022 pg/mL with a range of 1438 – 8126 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)

Interpolated concentration of native SynCAM/CADM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 25% plasma (citrate) 12.5% plasma (EDTA) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)

Dynamic range comparison between the Chemiluminescent Human SynCAM/CADM1 SimpleStep ELISA, colorimetric Human SynCAM/CADM1 SimpleStep ELISA (ab316889), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Lab

Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)

Standard curve comparison between the Chemiluminescent Human SynCAM/CADM1 SimpleStep ELISA and colorimetric Human SynCAM/CADM1 SimpleStep ELISA (ab316889) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Luminescent

サンプルタイプ

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

交差種

Human, Monkey

アッセイタイプ

Sandwich

Quantitative

検出感度

= 5.72 pg/mL

検出範囲

9.14 - 20000 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (ab326741) is a rapid single-wash sandwich 55-min ELISA to measure Human SynCAM/CADM1 in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma with a sensitivity of 5.72 pg/mL.

How the assay works

Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (ab326741) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SynCAM/CADM1 (ab326741) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-SynCAM/CADM1 antibody [EPR25965-58] - BSA and Aside free (Capture) ab308309
- Anti-SynCAM/CADM1 antibody [EPR25965-89] - BSA and Aside free (Detector) ab308310

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" } ]

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回収率

[ { "sample": "Serum", "range": "88 - 93 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "97 - 100 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "90 - 95 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "92 - 100 %", "average": "= 96" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner (PubMed : 12050160, PubMed : 22438059). Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner (By similarity). Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed : 15811952). In mast cells, may mediate attachment to and promote communication with nerves (PubMed : 15905536). CADM1, together with MITF, is essential for development and survival of mast cells in vivo (PubMed : 22438059). By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node (By similarity). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (By similarity). Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (By similarity). Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons (By similarity). May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa (By similarity). Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells (PubMed : 11279526, PubMed : 12234973). May contribute to the less invasive phenotypes of lepidic growth tumor cells (PubMed : 12920246).. Isoform 5. (Microbial infection) Induces cell fusion in neuron infected by a neuropathogenic strain of measles. Interacts with measles hemagglutinin to trigger hyperfusogenic F-mediated membrane fusion and presumably transsynaptic cell-to-cell transmission of the virus.

See full target information CADM1

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websiteProtocolBooklet

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