AB309312

Human Superoxide Dismutase 1 ELISA Kit

SimpleStep
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Human Superoxide Dismutase 1 ELISA Kit is a Cell-based ELISA for the measurement of Human Superoxide Dismutase 1 in Human in Cell/Tissue Extracts samples.

別名を表示する

Superoxide dismutase [Cu-Zn], Hydrogen sulfide oxidase, Superoxide dismutase 1, hSod1, SOD1

3 Images

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB309312)

sELISA

Lab

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB309312)

Interpolated concentrations of human Superoxide Dismutase 1 in HeLa cell extract and human liver tissue extract

Interpolated concentration of native Superoxide Dismutase 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 25μg/mL HeLa extract and 2μg/mL human liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB309312)

Interpolated concentrations of human Superoxide Dismutase 1 in HeLa cell extract and human liver tissue extract

sELISA

Lab

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB309312)

Example of human Superoxide Dismutase 1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Extracts, Cell Lysate

交差種

Human

アッセイタイプ

Cell-based

Quantitative

検出感度

= 0.468 ng/mL

検出範囲

2.344 - 150 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Superoxide Dismutase 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Superoxide Dismutase 1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Superoxide Dismutase 1 with 0.468 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" } ]

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回収率

[ { "sample": "Cell Lysate", "range": "93 - 105 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "114 - 118 %", "average": "= 116" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.

Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.

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製品プロトコール

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ターゲットの情報

Destroys radicals which are normally produced within the cells and which are toxic to biological systems (PubMed : 24140062). Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides (PubMed : 36630448).

See full target information SOD1

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websiteProtocolBooklet

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Abcam product promise

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Human Superoxide Dismutase 1 ELISA Kit