AB263890

Human SLPI ELISA Kit (ALP)

Recombinant
SimpleStep
詳細を見る

Be the first to review this product! Submit a review

(2 Publications)

Human SLPI ELISA Kit (ALP) is a single-wash 90-min Simplestep used to quantify Human SLPI (ALP) with a sensitivity of 5.59 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

WAP4, WFDC4, SLPI, Antileukoproteinase, ALP, BLPI, HUSI-1, Mucus proteinase inhibitor, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four-disulfide core domain protein 4, MPI

4 Images

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

The SLPI standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 62.6 ng/mL with a range of 41.2 – 85 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

Interpolated concentrations of native SLPI in human serum, plasma and urine samples.

Undiluted samples are as follows : serum 3%, plasma (citrate) 3%, plasma (EDTA) 3%, plasma (heparin) 3% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 55.8 ng/mL in neat serum, 40.9 ng/mL in neat plasma (citrate), 46.4 ng/mL in neat plasma (EDTA), 31.7 ng/mL in neat plasma (heparin) and 2.43 ng/mL in neat urine.

sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

Human SLPI standard curve comparison.

Standard Curve comparison between human SLPI SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

検出方法

Colorimetric

サンプルタイプ

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 5.59 pg/mL

検出範囲

31.25 - 2000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Human SLPI ELISA Kit (ALP) (ab263890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SLPI (ALP) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SLPI (ALP) with 5.59 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SLPI (Secretory Leukocyte Protease Inhibitor), also known as ALP (Antileukoproteinase) is acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. It modulates the inflammatory and immune responses after bacterial and parasite infection.

style

left-column

精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

style

left-column

回収率

[ { "sample": "Serum", "range": "109 - 123 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "98 - 151 %", "average": "= 119" }, { "sample": "Urine", "range": "99 - 109 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "96 - 120 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "108 - 119 %", "average": "= 113" } ]

style

left-column

製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263890-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB263890-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB263890-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263890-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263890-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263890-CMP05", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263890-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263890-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SLPI (ALP) Lyophilized Recombinant Protein", "number":"AB263890-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SLPI (ALP) Capture Antibody", "number":"AB263890-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SLPI (ALP) Detector Antibody", "number":"AB263890-CMP02", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Secretory leukocyte protease inhibitor (SLPI) also known as antileukoproteinase and neutrophil elastase inhibitor is a protein with a molecular mass of approximately 12 kDa. SLPI is found mainly in epithelial tissues and is expressed in locations such as the respiratory tract skin and cervix. Mechanically SLPI functions as a serine protease inhibitor binding to and neutralizing enzymes like neutrophil elastase cathepsin G and trypsin which helps to limit tissue damage during inflammation.

Biological function summary

SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.

Pathways

SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.

SLPI is implicated in conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis where excessive protease activity leads to tissue damage. In these diseases SLPI shows a protective role against lung tissue degradation. SLPI is also associated with the progression of certain cancers like ovarian cancer where its overexpression may support tumor growth and metastasis. In these contexts SLPI relates to other protease inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) due to their shared function in regulating protease activity and tissue remodeling.

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed : 10702419, PubMed : 2039600, PubMed : 2110563, PubMed : 24121345, PubMed : 3462719, PubMed : 3533531). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (By similarity). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed : 10702419, PubMed : 24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (By similarity). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed : 24352879).

See full target information SLPI

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:23013 PubMed38155270

2023

Recombinant human secretory leukocyte protease inhibitor (rhSLPI) coated titanium enhanced human osteoblast adhesion and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Radchanon Leelasukseree,Wannapat Chouyratchakarn,Chayanisa Phutiyothin,Faprathan Pikwong,Onnicha Srisopar,Phornsawat Baipaywad,Suruk Udomsom,Podsawee Mongkolpathumrat,Chayarop Supanchart,Sarawut Kumphune

Journal of immunology research 2021:1057186 PubMed33506054

2021

Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Yanran Huang,Ming Wang,Yu Hong,Xiangting Bu,Ge Luan,Yang Wang,Ying Li,Hongfei Lou,Chengshuo Wang,Luo Zhang

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SLPI ELISA Kit (ALP)