AB272477

Human SIRP alpha ELISA Kit (CD172a)

Recombinant
SimpleStep
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Human SIRP alpha ELISA Kit (CD172a) is a single-wash 90-min Simplestep used to quantify Human SIRP alpha (CD172a) with a sensitivity of 76.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3

4 Images

ELISA - Human SIRP alpha ELISA Kit (CD172a) (AB272477)

ELISA

Supplier Data

ELISA - Human SIRP alpha ELISA Kit (CD172a) (AB272477)

sELISA

Supplier Data

Sandwich ELISA - Human SIRP alpha ELISA Kit (CD172a) (AB272477)

Example of human SIRP alpha standard curve in 1X Cell Extraction Buffer PTR

sELISA

Supplier Data

Sandwich ELISA - Human SIRP alpha ELISA Kit (CD172a) (AB272477)

Representative image of Human SIRP alpha ELISA Kit (CD172a) (ab272477)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human SIRP alpha ELISA Kit (CD172a) Capture Antibody

- 10X Human SIRP alpha (CD172a) Detector Antibody

- 10X Human SIRP alpha ELISA Kit (CD172a) Lyophilised Purified Protein (2 vials)

- Human SIRP alpha ELISA Kit (CD172a) 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human SIRP alpha ELISA Kit (CD172a) (AB272477)

Interpolated concentrations of native SIRP alpha in human U-937 cell extract.

based on a 250 μg/mL extract load, SH-SY5Y cell extract based on a 2,000 μg/mL extract load, and brain tissue extract based on a 10 μg/mL extract load The concentrations of SIRP alpha were measured in duplicate and interpolated from the SIRP alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SIRP alpha concentration was determined to be 7.08 ng/mL in U-937 extract and 3.03 ng/mL in SH-SY5Y extract, and 6.85 ng/mL in brain extract.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Extracts, Cell Lysate

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 76.9 pg/mL

検出範囲

156.25 - 10000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human SIRP alpha ELISA Kit (CD172a) ab272477 is a rapid single-wash 90-min Sandwich ELISA to measure Human SIRP alpha (CD172a)in cell lysate, tissue extracts. This SimpleStep sensitivity is 76.9 pg/mL.

How the assay works

Human SIRP alpha (CD172a)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SIRP alpha ELISA Kit (CD172a) ab272477 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

SIRP alpha (SIPRA or CD127a) is a 37.73 kD immunoglobulin-like cell surface receptor for CD47. SIRP alpha is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors, or insulin. SIRP alpha may play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity.) The antibodies are designed to a fragment of the extracellular domain of SIRP alpha.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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回収率

[ { "sample": "Tissue Extracts", "range": "87 - 90 %", "average": "= 88" }, { "sample": "Cell Lysate", "range": "91 - 105 %", "average": "= 99" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.

Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.

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製品プロトコール

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ターゲットの情報

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed : 24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed : 24511121).

See full target information SIRPA

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websiteProtocolBooklet

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Abcam product promise

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Human SIRP alpha ELISA Kit (CD172a)