Human Siglec 9 ELISA Kit (CD329) (ab113339)
Key features and details
- Sensitivity: 130 pg/ml
- Range: 0.123 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Siglec 9 ELISA Kit (CD329) -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 130 pg/ml -
検出範囲
0.123 ng/ml - 30 ng/ml -
添加回収試験
85 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95.43 88% - 102% Serum 82.7 72% - 91% Plasma 78.16 70% - 91% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human Siglec 9 2 vials Recombinant Human Siglec 9 Standard (lyophilized) 2 vials Siglec 9 Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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参照データベース
- SwissProt: Q9Y336 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Representative Standard Curve
Representative Standard Curve using ab113339
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Representative Standard CurveRepresentative Standard Curve using ab113339
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113339 は論文での使用が確認できていません。