Human Anti-Schistosoma mansoni IgG ELISA Kit (ab108769)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Human Anti-Schistosoma mansoni IgG ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Pos. Serum 24 5.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Pos. Serum 4 5.7% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Schistosoma mansoni IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma.
A 96-well plate has been precoated with Schistosoma mansoni antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidise (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Schistosoma mansoni-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Schistosoma mansoni IgG sample captured in plate.
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アプリケーション
適用あり: ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Protein A HRP Conjugate Blue, Black cap 1 x 20ml Schistosoma mansoni (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Schistosoma mansoni IgG Cut-off Control Yellow, Green cap 1 x 3ml Schistosoma mansoni IgG Negative Control Yellow, Blue cap 1 x 2ml Schistosoma mansoni IgG Positive Control Yellow, Red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108769の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
画像
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108769 は 1 報の論文で使用されています。
- Huettner I et al. Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans. Cell Rep 38:110611 (2022). PubMed: 35354052