AB137970

Human SAP ELISA Kit (APCS)

Name: Human SAP ELISA Kit (APCS) (ab137970) | Abcam
Brand: Abcam Limited
SKU: AB137970
Price: 109000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Human SAP ELISA Kit (APCS) is a Sandwich ELISA for the measurement of Human SAP (APCS) in Human in Biofluids samples.

別名を表示する

PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein

2 Images

Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)

sELISA

Lab

Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)

SAP measured in various samples showing quantity (microgram) per mL of tested sample

sELISA

Supplier Data

Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)

Representative Standard Curve using ab137970.

Key facts

検出方法

Colorimetric

サンプルタイプ

Urine, Plasma, Serum, Cerebral Spinal Fluid

交差種

Human

アッセイタイプ

Sandwich

検出感度

> 24 pg/mL

検出範囲

0.156 - 10 ng/mL

アッセイ時間

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human SAP (APCS) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human SAP in plasma, serum, urine, and CSF.

A SAP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a SAP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of SAP captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]

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回収率

[ { "sample": "Serum", "range": "97 - 104 %", "average": null }, { "sample": "Plasma", "range": "88 - 109 %", "average": null } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137970-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137970-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137970-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137970-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SAP Microplate (12 x 8 well strips)", "number":"AB137970-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB137970-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137970-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human SAP Antibody", "number":"AB137970-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"SAP Standard", "number":"AB137970-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137970-CMP03", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.

Biological function summary

SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.

Pathways

SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.

SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.

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製品プロトコール

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ターゲットの情報

Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.

See full target information APCS

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

RSC advances 8:10598-10609 PubMed35540476

2018

Identification of potential serum biomarkers of acute paraquat poisoning in humans using an iTRAQ quantitative proteomic.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Wei,Yi Wang,Ling Lin,Lei Zhang,Yan Shi,Ping Xiang,Shujun Cao,Min Shen,Pengyuan Yang

Proteomics. Clinical applications 11: PubMed28261998

2017

Use of a Targeted Urine Proteome Assay (TUPA) to identify protein biomarkers of delayed recovery after kidney transplant.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth R Williams,Christopher M Colangelo,Lin Hou,Lisa Chung,Justin M Belcher,Thomas Abbott,Isaac E Hall,Hongyu Zhao,Lloyd G Cantley,Chirag R Parikh

View all publications

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websiteProtocolBooklet

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Human SAP ELISA Kit (APCS)