Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)
Key features and details
- Sensitivity: 8.3 ng/ml
- Range: 12.5 ng/ml - 800 ng/ml
- Sample type: Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Retinol binding protein 4 ELISA Kit (RBP4)
RBP4 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 3.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 8.5% -
サンプルの種類
Saliva, Milk, Urine, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 8.3 ng/ml -
検出範囲
12.5 ng/ml - 800 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Retinol binding protein 4 (RBP4) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein 4 concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.
A Retinol binding protein 4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein 4specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein 4 captured in plate.
Get higher sensitivity in only 90 minutes with Human Retinol Binding Protein 4 ELISA Kit (ab196264) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human Retinol binding Protein 4 Antibody 1 x 150µl Chromogen Substrate 1 x 7ml Retinol binding Protein 4 Microplate (12 x 8 well strips) 1 unit Retinol binding Protein 4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. -
関連疾患
Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- OTTHUMP00000020114
- OTTHUMP00000020115
- OTTHUMP00000020116
see all -
参照データベース
- Entrez Gene: 5950 Human
- Omim: 180250 Human
- SwissProt: P02753 Human
- Unigene: 50223 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108897の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve Using ab108897.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108897 は 5 報の論文で使用されています。
- Torabi R & Ghourchian H Ultrasensitive nano-aptasensor for monitoring retinol binding protein 4 as a biomarker for diabetes prognosis at early stages. Sci Rep 10:594 (2020). PubMed: 31953481
- Tayebi SM et al. Plasma retinol-binding protein-4 and tumor necrosis factor-a are reduced in postmenopausal women after combination of different intensities of circuit resistance training and Zataria supplementation. Sport Sci Health 15:551-558 (2019). PubMed: 31885723
- Al-Attar A et al. Data correlations between gender, cytomegalovirus infection and T cells, NK cells, and soluble immune mediators in elderly humans. Data Brief 8:536-44 (2016). PubMed: 27508213
- Gonzalez-Calero L et al. Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis? Sci Rep 6:19667 (2016). PubMed: 26792617
- Pirazzi C et al. PNPLA3 has retinyl-palmitate lipase activity in human hepatic stellate cells. Hum Mol Genet N/A:N/A (2014). PubMed: 24670599