AB325076

Human Ret ELISA Kit

SimpleStep
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Human Ret ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Ret with a sensitivity of 0.044ng/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

別名を表示する

CDHF12, CDHR16, PTC, RET, RET51, Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret

4 Images

Sandwich ELISA - Human Ret ELISA Kit (AB325076)

sELISA

Lab

Sandwich ELISA - Human Ret ELISA Kit (AB325076)

Example of human Ret standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Ret ELISA Kit (AB325076)

Interpolated concentration of native Ret was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL SH-SY5Y cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human Ret ELISA Kit (AB325076)

Interpolated concentration of native Ret was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL SH-SY5Y cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human Ret ELISA Kit (AB325076)

Example of human Ret standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Extract

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.044 ng/mL

検出範囲

0.19 - 12 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Ret ELISA Kit (ab325076) is a rapid single-wash sandwich 90-min ELISA to measure Human Ret in Cell Extract. This SimpleStep sensitivity is 0.044 ng/ml.

How the assay works

Human Ret ELISA Kit (ab325076) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Ret (ab325076) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human Ret ELISA Kit only requires 12.5µL sample.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" } ]

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回収率

[ { "sample": "Cell Lysate", "range": "103 - 122 %", "average": "= 110" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab325076-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB325076-CMP13" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325076-CMP11" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB325076-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB325076-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325076-CMP07" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325076-CMP06" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325076-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB325076-CMP04" }, { "size":"20 x 1 Vial", "name":"Human Ret Lyophilized Recombinant Protein", "number":"AB325076-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Ret Detector Antibody", "number":"AB325076-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Ret Capture Antibody", "number":"AB325076-CMP01" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab325076-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325076-CMP13" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB325076-CMP10" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325076-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB325076-CMP12" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB325076-CMP09" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325076-CMP06" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325076-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB325076-CMP04" }, { "size":"2 x 1 Vial", "name":"Human Ret Lyophilized Recombinant Protein", "number":"AB325076-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human Ret Detector Antibody", "number":"AB325076-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human Ret Capture Antibody", "number":"AB325076-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325076-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325076-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325076-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB325076-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB325076-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325076-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325076-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325076-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325076-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB325076-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Ret Lyophilized Recombinant Protein", "number":"AB325076-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ret Detector Antibody", "number":"AB325076-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ret Capture Antibody", "number":"AB325076-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15) (PubMed : 20064382, PubMed : 20616503, PubMed : 20702524, PubMed : 21357690, PubMed : 21454698, PubMed : 24560924, PubMed : 28846097, PubMed : 28846099, PubMed : 28953886, PubMed : 31118272). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation (PubMed : 21994944, PubMed : 23333276, PubMed : 28846097, PubMed : 28846099, PubMed : 28953886). GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways (PubMed : 21994944, PubMed : 23333276, PubMed : 24560924, PubMed : 25242331, PubMed : 28846097, PubMed : 28846099, PubMed : 28953886). Acts as a dependence receptor via the GDNF-GFRA1 signaling : in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF (PubMed : 20616503, PubMed : 21994944). Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling : involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PubMed : 28846097, PubMed : 28846099, PubMed : 28953886). Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner (PubMed : 20702524, PubMed : 21357690). Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage (PubMed : 21357690). Triggers the differentiation of rapidly adapting (RA) mechanoreceptors (PubMed : 20064382). Involved in the development of the neural crest (By similarity). Regulates nociceptor survival and size (By similarity). Phosphorylates PTK2/FAK1 (PubMed : 21454698).. Isoform 1. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.

See full target information RET

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websiteProtocolBooklet

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Abcam product promise

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Human Ret ELISA Kit