AB325080

Human Regucalcin ELISA Kit

SimpleStep
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Human Regucalcin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Regucalcin with a sensitivity of 26.24pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders.

別名を表示する

SMP30, RGN, Regucalcin, RC, Gluconolactonase, Senescence marker protein 30, GNL, SMP-30

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Sandwich ELISA - Human Regucalcin ELISA Kit (AB325080)

sELISA

Lab

Sandwich ELISA - Human Regucalcin ELISA Kit (AB325080)

Example of human Regucalcin standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

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Sandwich ELISA - Human Regucalcin ELISA Kit (AB325080)

Interpolated concentration of native Regucalcin was measured in duplicate at different sample concentrations. Undiluted samples are 250 µg/mL HepG2 cell extract and 62.5 µg/mL human liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Extract, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 26.24 pg/mL

検出範囲

117.188 - 7500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Regucalcin ELISA Kit (ab325080) is a rapid single-wash sandwich 90-min ELISA to measure Human Regucalcin in Cell Extract, Tissue Extract. This SimpleStep sensitivity is 26.24 pg/ml.

How the assay works

Human Regucalcin ELISA Kit (ab325080) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Regucalcin (ab325080) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.5" } ]

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回収率

[ { "sample": "Cell Lysate", "range": "94 - 101 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "90 - 100 %", "average": "= 94" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab325080-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB325080-CMP12" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325080-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB325080-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB325080-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325080-CMP06" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325080-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325080-CMP04" }, { "size":"20 x 1 Vial", "name":"Human Regucalcin Lyophilized Recombinant Protein", "number":"AB325080-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Regucalcin Detector Antibody", "number":"AB325080-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Regucalcin Capture Antibody", "number":"AB325080-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325080-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325080-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325080-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB325080-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB325080-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325080-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325080-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325080-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325080-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Regucalcin Lyophilized Recombinant Protein", "number":"AB325080-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Regucalcin Detector Antibody", "number":"AB325080-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Regucalcin Capture Antibody", "number":"AB325080-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).

See full target information RGN

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websiteProtocolBooklet

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Abcam product promise

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Human Regucalcin ELISA Kit