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リコンビナントSimpleStep

Human Rab3A ELISA Kit (ab300319)

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Example of human Rab3A standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of human Rab3A in brain cortex extract and brain stem extract
  • Human Rab3A ELISA Kit (ab300319)
  • Human Rab3A ELISA Kit (ab300319)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 39.195 pg/ml
  • Range: 140.625 pg/ml - 9000 pg/ml
  • Sample type: Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human Rab3A ELISA Kit
    Rab3A キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Extract 8 4.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Extract 3 5%

  • サンプルの種類

    Tissue Extracts

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    39.195 pg/ml

  • 検出範囲

    140.625 pg/ml - 9000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Tissue Extracts 102 87% - 113%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human Rab3A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rab3A protein in human tissue extract. Quantitate Human Rab3A with 64.254 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 96 tests
    10X Human Rab3A Capture Antibody 1 x 600µl
    10X Human Rab3A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Human Rab3A Lyophilized Recombinant Protein 2 vials
    Plate Seal 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

  • 組織特異性

    Specifically expressed in brain.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 別名

    • Rab 3A
    • RAB 3A member RAS oncogene family
    • Rab3a
    • RAB3A member RAS oncogene family
    • RAB3A_HUMAN
    • RAS associated protein RAB 3A
    • RAS associated protein RAB3A
    • Ras related protein Rab 3A
    • Ras related protein Rab3A
    • Ras-related protein Rab-3A
    see all

  • 参照データベース

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab300319の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Example of human Rab3A standard curve in 1X Cell Extraction Buffer PTR
      Example of human Rab3A standard curve in 1X Cell Extraction Buffer PTR
      Example of human Rab3A standard curve. Background-subtracted data values (mean +/- SD) are graphed
    • Interpolated concentrations of human Rab3A in brain cortex extract and brain stem extract
      Interpolated concentrations of human Rab3A in brain cortex extract and brain stem extract
    • Human Rab3A ELISA Kit (ab300319)
      Human Rab3A ELISA Kit (ab300319)
    • Human Rab3A ELISA Kit (ab300319)
      Human Rab3A ELISA Kit (ab300319)

    参考文献 (0)

    ab300319 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300319 は論文での使用が確認できていません。

    レビューと Q&A

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