AB230931

Human PTH ELISA Kit

Recombinant
SimpleStep
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Human PTH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PTH with a sensitivity of 0.761 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

別名を表示する

Parathyroid hormone, PTH, Parathormone, Parathyrin

4 Images

ELISA

Supplier Data

ELISA - Human PTH ELISA Kit (AB230931)

sELISA

Supplier Data

Sandwich ELISA - Human PTH ELISA Kit (AB230931)

Interpolated concentrations of spiked PTH in Human serum, plasma and cell culture media samples.

The concentrations of PTH were measured in duplicate, interpolated from the PTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50% and 10F RPMI media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTH concentration was determined to be 262.33 pg/mL in serum, 258.51 pg/mL in plasma (citrate) and 264.22 pg/mL in plasma (EDTA), 249.32 pg/mL in plasma (heparin), and 300.25 pg/mL in 10F RPMI media 100%.

sELISA

Lab

Sandwich ELISA - Human PTH ELISA Kit (AB230931)

Example of Human PTH standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PTH ELISA Kit (AB230931)

Example of Human PTH standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.761 pg/mL

検出範囲

4.69 - 300 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human PTH ELISA Kit ab230931 is a rapid single-wash 90-min Sandwich ELISA to measure Human PTH in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.761 pg/mL.

How the assay works

Human PTH SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PTH ELISA Kit ab230931 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human PTH ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Parathyroid Hormone antibody [EPR9939-66] - BSA and Azide free (Capture) ab244971
- Anti-Parathyroid Hormone antibody [EPR20253-236] - BSA and Azide free (Detector) ab244971

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" } ]

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回収率

[ { "sample": "Serum", "range": "88 - 89 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "88 - 92 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "81 - 84 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "81 - 82 %", "average": "= 82" }, { "sample": "Cell culture media", "range": "104 - 106 %", "average": "= 105" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.

Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.

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製品プロトコール

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ターゲットの情報

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion (PubMed : 11604398, PubMed : 35932760). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 18375760, PubMed : 35932760). Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed : 21076856).

See full target information Parathyroid hormone

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文献 (8)

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JCI insight 9: PubMed38780544

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Metabolism of parathyroid organoids.

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Konjeti R Sekhar,Simona G Codreanu,Olivia C Williams,Jeffrey C Rathmell,W Kimryn Rathmell,John A McLean,Stacy D Sherrod,Naira Baregamian

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Production of parathyroid-like cells from thyroid stem cells in co-culture environment.

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Özge Karabiyik Acar,Gülçin Delal Nozhatzadeh,Alperen Tuncer,Gamze Torun Köse,Ezgi Hacihasanoğlu,Fikrettin Sahin,Erhan Aysan

Nature communications 11:6249 PubMed33288743

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Cytosolic sequestration of the vitamin D receptor as a therapeutic option for vitamin D-induced hypercalcemia.

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Daniela Rovito,Anna Y Belorusova,Sandra Chalhoub,Anna-Isavella Rerra,Elvire Guiot,Arnaud Molin,Agnès Linglart,Natacha Rochel,Gilles Laverny,Daniel Metzger

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2020

High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss.

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Ok-Hee Kim,Carmen J Booth,Han Seok Choi,Jinwook Lee,Jinku Kang,June Hur,Woo Jin Jung,Yun-Shin Jung,Hyung Jin Choi,Hyeonjin Kim,Joong-Hyuck Auh,Jung-Wan Kim,Ji-Young Cha,Young Jae Lee,Cheol Soon Lee,Cheolsoo Choi,Yun Jae Jung,Jun-Young Yang,Seung-Soon Im,Dae Ho Lee,Sun Wook Cho,Young-Bum Kim,Kyong Soo Park,Young Joo Park,Byung-Chul Oh

PloS one 14:e0219109 PubMed31557191

2019

Circulating levels of free 25(OH)D increase at the onset of rheumatoid arthritis.

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Vidyanand Anaparti,Xiaobo Meng,Mahadevappa Hemshekhar,Irene Smolik,Neeloffer Mookherjee,Hani El-Gabalawy

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A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher‑Neuhäuser syndrome.

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Ruizhi Zheng,Yaguang Zhao,Jiayu Wu,Yuanmei Wang,Jian-Ling Liu,Zhi-Ling Zhou,Xiao-Tao Zhou,Dan-Na Chen,Wei-Hua Liao,Jia-Da Li

View all publications

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websiteProtocolBooklet

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Human PTH ELISA Kit