Human PMN Elastase ELISA Kit

製品の詳細

Human PMN Elastase ELISA Kit ab119553 is a sandwich ELISA to measure Human PMN Elastase in serum, plasma with a sensitivity of 1.98 pg/ml.

How the assay works

PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.
The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human PMN Elastase ELISA Kit ab119553 protocol summary

1. Wash plate 2 times before adding standard, sample and control wells
2. Add each standards, controls or samples into appropriate wells. Incubate at room temperature
3. Wash
4. Add HRP Conjugated Antibody to all wells. Incubate at room temperature
5. Wash
6. Add TMB Substrate Solution to all wells. Incubate at room temperature
7. Add stop solution into each well
7. Read at 450 nm