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SimpleStep

Human PLTP ELISA Kit (ab289907)

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Example of human PLTP standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of human PLTP in U87 extract, JAR extract, and PC-3 extract.
  • Sandwich ELISA - Human PLTP ELISA Kit (ab289907)
  • Human PLTP ELISA Kit (ab289907)
  • Sandwich ELISA - Human PLTP ELISA Kit (ab289907)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 46.254 pg/ml
  • Range: 125 pg/ml - 8000 pg/ml
  • Sample type: Cell Lysate
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human PLTP ELISA Kit
    PLTP キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Extracts 8 11.4%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Extracts 3 11.2%

  • サンプルの種類

    Cell Lysate

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    >= 46.254 pg/ml

  • 検出範囲

    125 pg/ml - 8000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell Lysate 102 83% - 112%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human PLTP ELISA Kit (ab289907) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PLTP in cell lysate samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PLTP with 46.254 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289907の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration.
特記事項
Sandwich ELISA
Use at an assay dependent concentration.

画像

  • Example of human PLTP standard curve in 1X Cell Extraction Buffer PTR.
    Example of human PLTP standard curve in 1X Cell Extraction Buffer PTR.

    Example of human PLTP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of human PLTP in U87 extract, JAR extract, and PC-3 extract.
    Interpolated concentrations of human PLTP in U87 extract, JAR extract, and PC-3 extract.

    Interpolated concentration of native PLTP was measured in duplicate at different sample concentrations. Undiluted samples are as follows: U87 extract 31.25 μg/mL, JAR extract 62.5 μg/mL, PC-3 extract 62.5 μg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

  • Sandwich ELISA - Human PLTP ELISA Kit (ab289907)
    Sandwich ELISA - Human PLTP ELISA Kit (ab289907)
    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
  • Human PLTP ELISA Kit (ab289907)
    Human PLTP ELISA Kit (ab289907)
  • Sandwich ELISA - Human PLTP ELISA Kit (ab289907)
    Sandwich ELISA - Human PLTP ELISA Kit (ab289907)
    To learn more about the advantages of SimpleStep ELISA® kits see here.

参考文献 (0)

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