Human Perforin ELISA Kit (PRF1) (ab46068)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 62.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Perforin ELISA Kit (PRF1) -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 8 416pg/ml 20 5% B 8 674pg/ml 25 4% C 8 984pg/ml 24 3% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A 9 580pg/ml 15 3% B 9 665pg/ml 31 5% C 9 1004pg/ml 45 5% -
サンプルの種類
Cell culture supernatant -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 40 pg/ml -
検出範囲
62.5 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 71 % - % -
全工程の試験時間
2h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Perforin (PRF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Perforin in supernatant and buffered solutions.
A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples.
This kit will recognize both endogenous and recombinant Human Perforin.
Please note this kit is not suitable for use with serum and plasma. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 1X Standard Diluent Buffer 2 x 25ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated anti PRF1 Red 2 x 0.4ml 1 x 0.4ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml PRF1 Microplate (12 x 8 well strips) 2 units 1 unit PRF1 Standard (Lyophilized) Yellow 4 vials 2 vials Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究分野
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機能
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. -
関連疾患
Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found. -
配列類似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 C2 domain.
Contains 1 EGF-like domain.
Contains 1 MACPF domain. -
ドメイン
The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. - Information by UniProt
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別名
- Cytolysin
- FLH2
- HPLH2
see all -
参照データベース
- Entrez Gene: 5551 Human
- Omim: 170280 Human
- SwissProt: P14222 Human
- Unigene: 2200 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46068の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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ELISA: Perforin (PRF1) Human ELISA Kit (ab46068)
Perforin measured in cell culture supernatants (tested at 1/1-1/100 dilution range; duplicates +/- SD).
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ELISA: Perforin (PRF1) Human ELISA Kit (ab46068)Standard curve with background signal subtracted (duplicates; +/- SD).
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Typical dataRepresentative standard curve using ab46068
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab46068 は 10 報の論文で使用されています。
- Capelle CM et al. Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females. Nat Commun 14:7461 (2023). PubMed: 37985656
- Zhong R et al. Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP. Sci Adv 9:eadj9964 (2023). PubMed: 38134285
- Chu Y et al. Combinatorial immunotherapy of N-803 (IL-15 superagonist) and dinutuximab with ex vivo expanded natural killer cells significantly enhances in vitro cytotoxicity against GD2+ pediatric solid tumors and in vivo survival of xenografted immunodeficient NSG mice. J Immunother Cancer 9:N/A (2021). PubMed: 34244307
- Sasawatari S et al. Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells. J Immunol 204:1373-1385 (2020). PubMed: 31969386
- Hu G et al. An IL6-Adenosine Positive Feedback Loop between CD73+ γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer. Cancer Immunol Res 8:1273-1286 (2020). PubMed: 32847938
- Sevim H et al. Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Stem Cell Res Ther 9:198 (2018). PubMed: 30021624
- Wang Y et al. NKG2D modulates aggravation of liver inflammation by activating NK cells in HBV infection. Sci Rep 7:88 (2017). PubMed: 28273905
- Sarkar S et al. Dynamic Analysis of Human Natural Killer Cell Response at Single-Cell Resolution in B-Cell Non-Hodgkin Lymphoma. Front Immunol 8:1736 (2017). ELISA . PubMed: 29312292
- Schreiner J et al. Expression of inhibitory receptors on intratumoral T cells modulates the activity of a T cell-bispecific antibody targeting folate receptor. Oncoimmunology 5:e1062969 (2016). PubMed: 27057429
- Freeman LM et al. Myeloma-induced alloreactive T cells arising in myeloma-infiltrated bones include double-positive CD8+CD4+ T cells: evidence from myeloma-bearing mouse model. J Immunol 187:3987-96 (2011). PubMed: 21908738