AB242246

Human PDGF R alpha ELISA Kit

Recombinant
SimpleStep
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Human PDGF R alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PDGF R alpha with a sensitivity of 1.89 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2

6 Images

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

sELISA

Unknown

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

Interpolated concentrations of native PDGF R alpha in human serum, and plasma samples. The concentrations of PDGF R alpha were measured in duplicates, interpolated from the PDGF R alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF R alpha concentration was determined to be 5932.99 pg/mL in serum, 5414.30 pg/mL in plasma (citrate), 5256.55 pg/mL in plasma (EDTA), and 4851.59 pg/mL plasma (heparin).

sELISA

Unknown

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

Example of human PDGF R alpha standard curve in Sample Diluent NS. The PDGF R alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

Representative image of Human PDGF R alpha ELISA Kit (ab242246)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human PDGF R alpha ELISA Kit Capture Antibody

- 10X Human PDGF R alpha Detector Antibody

- 10X Human PDGF R alpha ELISA Kit Lyophilised Purified Protein (2 vials)

- Human PDGF R alpha ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

Example of human PDGF R alpha standard curve in 1X Cell Extraction Buffer PTR. The PDGF R alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

Interpolated concentrations of native PDGF R alpha in human SH-SY5Y based on a 125 ug/mL load.

Interpolated concentrations of native PDGF R alpha in human placenta, and skeletal muscle extract samples based on a 250 μg/mL extract load. The concentrations of PDGF R alpha were measured in duplicate and interpolated from the PDGF R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF R alpha concentration was determined to be 1105.74 pg/mL in SH-SY5Y extract, 1507.92 pg/mL in placenta extract and 1521.98 pg/mL in skeletal muscle extract.

sELISA

Unknown

Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

Serum (10%) from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF R alpha concentration was determined to be 7160.36 pg/mL with a range of 4446.95 - 12638.24 pg/mL.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 1.89 pg/mL

検出範囲

31.25 - 2000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human PDGF R alpha ELISA Kit (ab242246) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF R alpha protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PDGF R alpha with 1.89 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "105 - 107 %", "average": "= 106" }, { "sample": "Serum", "range": "84 - 97 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "93 - 102 %", "average": "= 97" }, { "sample": "Cell culture extracts", "range": "85 - 97 %", "average": "= 90" }, { "sample": "Tissue Extracts", "range": "91 - 105 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "94 - 105 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "100 - 102 %", "average": "= 102" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242246-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242246-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242246-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242246-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB242246-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242246-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242246-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242246-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB242246-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PDGF R alpha Lyophilized recombinant Protein", "number":"AB242246-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PDGF R alpha Capture Antibody", "number":"AB242246-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PDGF R alpha Detector Antibody", "number":"AB242246-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB242246-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.

Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.

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製品プロトコール

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ターゲットの情報

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

See full target information PDGFRA

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websiteProtocolBooklet

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Human PDGF R alpha ELISA Kit