AB309187

Human OSMR ELISA Kit - Extracellular

SimpleStep
Recombinant
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Human OSMR ELISA Kit - Extracellular is a Sandwich ELISA for the measurement of Human OSMR - Extracellular in Human in Biofluids, Cell Culture Media samples.

別名を表示する

OSMRB, OSMR, Oncostatin-M-specific receptor subunit beta, Interleukin-31 receptor subunit beta, IL-31 receptor subunit beta, IL-31R subunit beta, IL-31R-beta, IL-31RB

3 Images

Sandwich ELISA - Human OSMR ELISA Kit - Extracellular (AB309187)

sELISA

Supplier Data

Sandwich ELISA - Human OSMR ELISA Kit - Extracellular (AB309187)

Interpolated concentrations of human OSMR in serum and plasma (EDTA)

Interpolated concentration of native human OSMR was measured in duplicate at different sample concentrations. Undiluted samples are 1% serum and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human OSMR ELISA Kit - Extracellular (AB309187)

Interpolated concentrations of human OSMR in Hela cell culture supernatant

Interpolated concentration of native human OSMR was measured in duplicate at different sample concentrations. Undiluted samples are 50% Hela cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human OSMR ELISA Kit - Extracellular (AB309187)

Example of human OSMR standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, EDTA Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 15.56 pg/mL

検出範囲

43.75 - 2800 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human OSMR - Extracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OSMR - Extracellular protein in human serum, EDTA plasma and cell culture supernatant. Quantitate Human OSMR - Extracellular with 15.56 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.12" } ]

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回収率

[ { "sample": "Serum", "range": "92 - 103 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "89 - 108 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "97 - 105 %", "average": "= 101" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309187-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309187-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309187-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309187-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309187-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309187-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB309187-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OSMR - Extracellular Capture Antibody", "number":"AB309187-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OSMR - Extracellular Lyophilized Recombinant Protein", "number":"AB309187-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309187-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OSMR - Extracellular Detector Antibody", "number":"AB309187-CMP07", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Oncostatin M receptor commonly known as OSMR is an important membrane-bound receptor involved in numerous cellular processes. It is part of the type I cytokine receptor family and often forms heterodimers with gp130 to mediate its functions. OSMR has a mass of approximately 115 kDa and predominantly expresses in tissues such as skin lung liver and the nervous system. Alternative names for OSMR include Oncostatin-M-specific receptor beta subunit and IL-31 receptor beta.

Biological function summary

OSMR plays a significant role in regulating inflammatory responses and cell growth. It is part of a receptor complex that upon activation triggers intracellular signaling cascades driven by cytokines like Oncostatin M and IL-31. OSMR involvement influences processes including acute phase responses hematopoiesis and nerve cell differentiation. Its action helps mediate the communication between cells to respond to external inflammatory signals effectively.

Pathways

OSMR is actively involved in the JAK/STAT signaling pathway and the MAPK pathway. In JAK/STAT OSMR collaborates with related proteins like gp130 and participates in promoting transcriptional activity of specific genes in response to cytokine signaling. OSMR’s engagement in the MAPK pathway influences cellular responses such as proliferation differentiation and apoptosis linking it with proteins such as MAP Kinases that further proceed this signaling cascade.

OSMR shows a connection with atopic dermatitis and various cancers. Aberrant OSMR signaling can lead to excessive inflammatory responses contributing to skin conditions like atopic dermatitis often involving IL-31 in the pathway. In cancer elevated OSMR expression correlates with tumorigenesis and progression establishing its interaction with oncogenic proteins in the STAT3 pathway which may promote malignant cell behavior. Through these disease interactions OSMR represents a potential therapeutic target worth exploring.

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製品プロトコール

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ターゲットの情報

Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events.

See full target information OSMR

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websiteProtocolBooklet

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Human OSMR ELISA Kit - Extracellular