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AB326398

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent

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Human Omentin (ITLN1) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Omentin (ITLN1) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16.46 - 36000 pg/ml with a sensitivity of 10.5pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

別名を表示する

INTL, ITLN, LFR, UNQ640/PRO1270, ITLN1, Intelectin-1, ITLN-1, Endothelial lectin HL-1, Galactofuranose-binding lectin, Intestinal lactoferrin receptor, Omentin

5 Images
Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Lab

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Lab

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Standard curve comparison between the Chemiluminescent Human Omentin SimpleStep ELISA and colorimetric Human Omentin SimpleStep ELISA (ab269545) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Lab

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Dynamic range comparison between the Chemiluminescent Human Omentin SimpleStep ELISA, colorimetric Human Omentin SimpleStep ELISA (ab269545), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Supplier Data

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Interpolated concentrations of native Omentin in human serum.

The concentrations of Omentin were measured in duplicates interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean target concentration was determined to be 159.4 ng/mL in serum.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Supplier Data

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Serum from eight individual healthy Omentin female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Omentin concentration was determined to be 190.6 ng/mL with a range of 97.4 – 311.8 ng/mL.

Key facts

検出方法

Luminescent

サンプルタイプ

Serum

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 10.5 pg/mL

検出範囲

16.46 - 36000 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (ab326398) is a rapid single-wash sandwich 55-min ELISA to measure Human Omentin (ITLN1) in Serum with a sensitivity of 10.5 pg/mL.

How the assay works

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (ab326398) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Omentin (ITLN1) (ab326398) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ITLN1 antibody [EPR20311-300] - BSA and Aside free (Capture) ab252674
- Anti-ITLN1 antibody [EPR20311-224] - BSA and Aside free (Detector) ab252615
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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.1" } ]
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回収率

[ { "sample": "Serum", "range": "80 - 109 %", "average": "= 99" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed : 11313366, PubMed : 26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed : 26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed : 26148048). Does not bind human glycans (PubMed : 26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed : 16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed : 16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed : 11747454, PubMed : 23921499).
See full target information ITLN1
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websiteProtocolBooklet
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