AB267568

Human NrCAM ELISA Kit

Name: Human NrCAM ELISA Kit (ab267568) | Abcam
Brand: Abcam Limited
SKU: AB267568
Price: 109000 JPY
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Human NrCAM ELISA Kit is a Sandwich ELISA for the measurement of Human NrCAM in Human in Biofluids, Cell Culture Media samples.

別名を表示する

KIAA0343, NRCAM, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, hBravo, Ng-CAM-related

1 Images

Sandwich ELISA - Human NrCAM ELISA Kit (AB267568)

sELISA

Supplier Data

Sandwich ELISA - Human NrCAM ELISA Kit (AB267568)

Human NrCAM ELISA kit (ab267568) Standard curves in Assay Diluent A and Assay Diluent D.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture media, Serum

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 0.3 ng/mL

検出範囲

0.328 - 80 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Human NrCAM ELISA Kit is designed for the quantitative determination of NRCAM in cell culture supernatants, plasma and serum samples.

ΔNote: Human NrCAM concentration is low in normal serum and plasma samples and may not be detectable in this assay.

This assay employs an antibody specific for human NrCAM coated on a 96-well plate. Standards and samples are pipetted into the wells and NrCAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human NrCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NrCAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style

left-column

精度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

style

left-column

回収率

[ { "sample": "Serum", "range": "68 - 143 %", "average": "= 108" }, { "sample": "Plasma", "range": "68 - 106 %", "average": "= 79" }, { "sample": "Cell culture media", "range": "99 - 133 %", "average": "= 115" } ]

style

left-column

製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267568-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267568-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267568-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human NrCAM coated Microplate (12 x 8 wells)", "number":"AB267568-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267568-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267568-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267568-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB267568-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human NrCAM Detection Antibody", "number":"AB267568-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267568-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NrCAM Standard (Lyophilized)", "number":"AB267568-CMP01", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.

Biological function summary

NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.

Pathways

NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.

NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.

See full target information NRCAM

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human NrCAM ELISA Kit