AB315429

Human NPTXR ELISA Kit

SimpleStep
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Human NPTXR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NPTXR with a sensitivity of 2.351 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Neuronal pentraxin receptor, NPTXR

3 Images

Sandwich ELISA - Human NPTXR ELISA Kit (AB315429)

sELISA

Supplier Data

Sandwich ELISA - Human NPTXR ELISA Kit (AB315429)

Interpolated concentrations of human NPTXR in serum, plasma (EDTA), plasma (citrate), and plasma (heparin)

Interpolated concentration of native NPTXR was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human NPTXR ELISA Kit (AB315429)

Interpolated concentrations of human NPTXR in cerebrospinal fluid

Interpolated concentration of native NPTXR was measured in duplicate at different sample concentrations. Undiluted samples are 0.5% cerebrospinal fluid. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human NPTXR ELISA Kit (AB315429)

Example of human NPTXR standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 2.351 pg/mL

検出範囲

7.813 - 500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human NPTXR SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NPTXR protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cerebral Spinal Fluid. Quantitate Human NPTXR with 2.351 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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回収率

[ { "sample": "Serum", "range": "91 - 107 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "101 - 114 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "83 - 104 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "101 - 115 %", "average": "= 107" }, { "sample": "Cerebral Spinal Fluid", "range": "84 - 102 %", "average": "= 94" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NPTXR also known as Neuronal Pentraxin Receptor is a protein involved in the synaptic organization. It has a molecular mass of approximately 65 kDa. NPTXR expresses mainly in the central nervous system including the brain and spinal cord. As a synaptic protein it plays a role in modulating neuron-neuron interactions and signaling pathways critical for the central nervous system's proper function.

Biological function summary

Neuronal pentraxin receptor contributes to synaptic plasticity and maintenance. It forms a complex with other pentraxin proteins like NPTX1 and NPTX2 facilitating the clustering of AMPA receptors at synapses. This interaction enhances excitatory synaptic transmission which is important for processes like learning and memory. NPTXR therefore plays a significant role in maintaining synaptic strength and integrity.

Pathways

The neuronal pentraxin receptor influences the glutamatergic signaling pathway. This pathway involves neurotransmitter glutamate and its receptors which include AMPA-type glutamate receptors at postsynaptic sites. NPTXR's interaction with AMPA receptors via NPTX1 and NPTX2 promotes receptor clustering critical for NMDA receptor phosphorylation. As a result this protein contributes to synaptic plasticity influencing learning and memory pathways in neurons.

NPTXR has implications in neurodegenerative diseases and schizophrenia. Altered synaptic plasticity and receptor clustering can lead to cognitive deficits frequently observed in Alzheimer's disease. Additionally studies suggest a potential link between NPTXR and schizophrenia where dysregulated synaptic organization impacts neurotransmission. NPTXR's association with AMPA receptors through other pentraxins implicates it in these disorders highlighting its significance as a therapeutic target.

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製品プロトコール

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ターゲットの情報

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

See full target information NPTXR

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websiteProtocolBooklet

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Human NPTXR ELISA Kit