AB314848

Human NPTX2 ELISA Kit - Extracellular

SimpleStep
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Human NPTX2 ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA used to quantify Human NPTX2 with a sensitivity of 12.422 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327868

別名を表示する

Neuronal pentraxin-2, NP2, Neuronal pentraxin II, NP-II, NPTX2

2 Images

Sandwich ELISA - Human NPTX2 ELISA Kit - Extracellular (AB314848)

sELISA

Supplier Data

Sandwich ELISA - Human NPTX2 ELISA Kit - Extracellular (AB314848)

Interpolated concentrations of human NPTX2 in cerebrospinal fluid, serum, and plasma (EDTA)

Interpolated concentration of native NPTX2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : cerebrospinal fluid (10%), serum (50%), and plasma (EDTA) (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human NPTX2 ELISA Kit - Extracellular (AB314848)

Example of human NPTX2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cerebral Spinal Fluid, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 12.422 pg/mL

検出範囲

40.625 - 2600 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human NPTX2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NPTX2 protein in Cerebral Spinal Fluid, Serum, EDTA Plasma. Quantitate Human NPTX2 with 12.422 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]

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回収率

[ { "sample": "Cerebral Spinal Fluid", "range": "102 - 109 %", "average": "= 106" }, { "sample": "Serum", "range": "104 - 109 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "102 - 112 %", "average": "= 108" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NPTX2 protein also known as neuronal pentraxin II or NARP weighs approximately 47 kDa. NPTX2 is expressed mostly in the brain especially in neurons. This protein belongs to the neuronal pentraxin family and plays a mechanical role in processes like synaptic plasticity. NPTX2 operates by binding to AMPA receptors influencing their surface expression.

Biological function summary

NPTX2 affects synaptic stabilization by participating in the formation and maintenance of synaptic connections. It forms complexes with AMPA receptor subunits promoting synapses that are more active and facilitating synaptic communication. NPTX2's involvement is essential for normal synaptic function reflecting its significance in neural signaling.

Pathways

NPTX2 plays a role in the glutamatergic signaling pathway an important pathway for synaptic transmission involving excitatory neurotransmitters. NPTX2 works alongside other proteins like GluR1 subunit in this pathway mediating synaptic responses. Through its actions in these pathways NPTX2 contributes to long-term potentiation a process related to learning and memory.

NPTX2 has links to neurological conditions such as Alzheimer’s disease and schizophrenia. In Alzheimer's NPTX2 dysregulation associates with synaptic dysfunction possibly involving interactions with beta-amyloid. In schizophrenia altered NPTX2 expression might contribute to glutamatergic signaling imbalances affecting cognitive function. These associations highlight NPTX2's importance in maintaining normal neural processes.

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製品プロトコール

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ターゲットの情報

Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).

See full target information NPTX2

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websiteProtocolBooklet

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Human NPTX2 ELISA Kit - Extracellular