AB317781

Human NOTUM ELISA Kit- Extracellular

SimpleStep
Recombinant
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Human NOTUM ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human NOTUM with a sensitivity of 20.375 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

OK/SW-CL.30, NOTUM, Palmitoleoyl-protein carboxylesterase NOTUM, hNOTUM

6 Images

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

Interpolated concentrations of NOTUM in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NOTUM concentration was determined to be 1,684 pg/mL with a range of 801 - 3733 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

Interpolated concentrations of human NOTUM in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native NOTUM was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

Interpolated concentrations of human NOTUM in HepG2 cell culture supernatant. Interpolated concentration of native NOTUM was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 40 HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

Example of human NOTUM standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

Example of human NOTUM standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Extracellular (AB317781)

Interpolated concentrations of human NOTUM in HepG2 cell culture supernatant in 96-well vs. 384-well plates. Interpolated concentration of native NOTUM was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 40 HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, EDTA Plasma, Cell culture supernatant, Heparin Plasma, Citrate plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 20.375 pg/mL

検出範囲

46.875 - 3000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human NOTUM SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NOTUM protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human NOTUM with 20.375 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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精度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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回収率

[ { "sample": "Serum", "range": "84 - 120 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "90 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "85 - 111 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "90 - 97 %", "average": "= 94" }, { "sample": "Cell culture supernatant", "range": "98 - 113 %", "average": "= 104" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NOTUM is a lipase-like enzyme that functions mechanically to remove lipid modifications specifically palmitoleate from the Wnt proteins. It is alternatively known as palmitoleoyl-protein carboxylesterase. The molecular mass of NOTUM is approximately 53 kDa. NOTUM is expressed mainly in tissues such as the liver kidneys and gut where it plays a role in regulating signaling pathways.

Biological function summary

NOTUM inhibits the Wnt signaling pathway by cleaving the lipid moiety attached to Wnt proteins making it inactive. NOTUM acts independently and is not part of larger protein complexes. This regulation of Wnt signaling is important for maintaining cellular processes such as cell proliferation differentiation and migration especially during development and tissue homeostasis.

Pathways

NOTUM primarily influences the Wnt signaling pathway. This pathway is significant for embryonic development and adult homeostasis. By inactivating Wnt proteins NOTUM indirectly affects other pathway components like β-catenin which is an important mediator in the Wnt pathway. NOTUM ensures the balance of Wnt signaling preventing overactivation that could lead to abnormal cell growth.

NOTUM's role in regulating Wnt signaling links it to conditions such as colorectal cancer and osteoporosis. In cancers overexpression of NOTUM can contribute to Wnt pathway dysregulation while its inhibition may present a therapeutic angle. In osteoporosis alterations in NOTUM activity can affect bone formation processes through its influence on Wnt-related proteins like LRP5/6. Understanding NOTUM's interactions in these diseases may provide insights into novel treatment strategies.

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製品プロトコール

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ターゲットの情報

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed : 25731175).

See full target information NOTUM

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websiteProtocolBooklet

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Human NOTUM ELISA Kit- Extracellular