AB227894

Human Niemann Pick C2 ELISA Kit

Recombinant
SimpleStep
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Human Niemann Pick C2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Niemann Pick C2 with a sensitivity of 2.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

HE1, NPC2, NPC intracellular cholesterol transporter 2, Epididymal secretory protein E1, Human epididymis-specific protein 1, Niemann-Pick disease type C2 protein, He1

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Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)

sELISA

Supplier Data

Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)

Example of Human Niemann Pick C2 standard curve Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)

Interpolated concentrations of native Niemann Pick C2 in Human cell and tissue extract samples.

The concentrations of Niemann Pick C2 were measured in duplicate, interpolated from the Niemann Pick C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell extract (100 mg/ml), Human Pancreas (100 mg/ml) and Human Placenta (100 mg/ml) extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Niemann Pick C2 concentration was determined to be 286.36 pg/mL in HepG2 extract, 345.68 pg/mL in Human Pancreas extract and 303.6 pg/mL in Human Placenta extract based on the 100 μg/mL load.

sELISA

Supplier Data

Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)

Example of Human Niemann Pick C2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)

Interpolated concentrations of native Niemann Pick C2 in Human HepG2 Supernatant (2.5%).

The concentrations of Niemann Pick C2 were measured in duplicate and interpolated from the Niemann Pick C2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Niemann Pick C2 concentration was determined to be 3288.10 pg/mL in HepG2 Supernatant.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 2.58 pg/mL

検出範囲

19 - 1200 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Niemann Pick C2 ELISA Kit (ab227894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Niemann Pick C2 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Niemann Pick C2 with 2.58 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Tissue Extracts", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Intra", "sample": "Tissue Extracts", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "91 - 98 %", "average": "= 95" }, { "sample": "Cell culture extracts", "range": "90 - 95 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "93 - 94 %", "average": "= 94" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Niemann Pick C2 protein (NPC2) is a small intracellular cholesterol transport protein weighing approximately 16 kDa. Alternately referred to as "NPC2 protein" or "NPC 293" it binds with lipids and is predominantly expressed in the lysosome. This protein plays a critical role in the intracellular lipid transport process. NPC2 forms part of a family of proteins tasked with moving cholesterol and other related lipids within lysosomal compartments.

Biological function summary

NPC2 protein facilitates the transfer of cholesterol from lysosomes to other parts of the cell. It operates in tandem with its partner protein Niemann Pick C1 (NPC1) to mediate the egress of cholesterol. The NPC2 protein docks to NPC1 within the lysosomal membrane forming an essential complex in lipid transport. This function is pivotal for the maintenance of cellular cholesterol homeostasis influencing various cellular and systemic functions linked to cholesterol metabolism.

Pathways

NPC2 protein participates prominently in the cholesterol efflux pathways. It associates with the NPC1 protein to regulate the movement of cholesterol from the lysosomal membrane to cytoplasmic areas where it can be utilized or stored. This pathway links NPC2 to significant biological processes like the homeostasis of lipoprotein-derived cholesterol influencing the SREBP pathway which governs lipid synthesis and uptake. Such interactions highlight NPC2's involvement in regulating cellular lipid balance.

NPC2 protein dysfunction notably links to Niemann-Pick disease type C a lipid storage disorder. Mutations in the genes encoding NPC2 or NPC1 disrupt cholesterol transportation leading to abnormal lipid accumulation and progressive neurodegeneration. Additionally this protein's dysregulation is indirectly connected to atherosclerosis as such disruptions can result in altered cellular cholesterol management highlighting its interaction with proteins involved in cardiovascular diseases.

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製品プロトコール

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ターゲットの情報

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed : 11125141, PubMed : 15937921, PubMed : 17018531, PubMed : 18772377, PubMed : 29580834). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed : 17018531, PubMed : 18772377, PubMed : 27238017). May bind and mobilize cholesterol that is associated with membranes (PubMed : 18823126). NPC2 binds cholesterol with a 1 : 1 stoichiometry (PubMed : 17018531). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed : 17018531). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).

See full target information NPC2

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websiteProtocolBooklet

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Human Niemann Pick C2 ELISA Kit