AB288171

Human NEFL ELISA Kit

Name: Human NEFL ELISA Kit (ab288171) | Abcam
Brand: Abcam Limited
SKU: AB288171
Price: 93000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
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Human NEFL ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NEFL with a sensitivity of 30.785 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

3 Images

ELISA

Supplier Data

ELISA - Human NEFL ELISA Kit (AB288171)

sELISA

Supplier Data

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)

Interpolated concentrations of spiked human NEFL in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Recombinant NEFL was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)

Example of human NEFL standard curve in Sample Diluent NS. Example of human NEFL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 30.785 pg/mL

検出範囲

93.75 - 6000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human NEFL ELISA Kit ab288171 is a rapid single-wash 90-min Sandwich ELISA to measure Human NEFL in EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 30.785 pg/mL.

How the assay works

Human NEFL SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human NEFL ELISA Kit ab288171 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NEFL antibody [EPR23733-137] - BSA and Azide free (Capture) ab280028
- Anti-NEFL antibody [EPR23733-154] - BSA and Azide free (Detector) ab280028

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]

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回収率

[ { "sample": "Serum", "range": "113 - 118 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "99 - 117 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "113 - 116 %", "average": "= 115" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288171-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288171-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288171-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288171-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NEFL Lyophilized Recombinant Protein", "number":"AB288171-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NEFL Capture Antibody", "number":"AB288171-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB288171-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human NEFL Detector Antibody", "number":"AB288171-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288171-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288171-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288171-CMP06", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NEFL also known as neurofilament light polypeptide is a component of the cytoskeleton of neurons. It is one of the subunits of neurofilaments which also include medium and heavy chains. NEFL has a molecular mass of about 61.5 kDa. The protein is predominantly expressed in neurons throughout the central and peripheral nervous systems providing structural support and regulating axon diameter.

Biological function summary

Neurofilament light polypeptide forms heteropolymers with other neurofilament proteins contributing to the assembly of stable neurofilament fibers. These fibers maintain neuronal integrity and proper conductance velocities of nerve signals. NEFL interacts closely with neurofilament medium and heavy chains playing a role in the structural framework of the neuron. This enables efficient transmission of electrical impulses along axons.

Pathways

NEFL plays a significant role in the neurofilament pathway which is critical for neuronal cytoskeleton assembly and axonal transport. This pathway involves proteins such as NEFM (neurofilament medium) and NEFH (neurofilament heavy). NEFL's involvement in maintaining axonal caliber connects it to important signaling cascades like the axon guidance and neurotransmitter release pathways enhancing neuronal communication.

Defects in NEFL can lead to neurological conditions such as Charcot-Marie-Tooth disease type 2E and amyotrophic lateral sclerosis. Mutations or disruptions in NEFL impact axonal transport and neurofilament assembly contributing to neurodegeneration. NEFL is often studied alongside proteins like peripherin which can also exhibit mutations linked to similar neuropathies underlining its importance in neural health.

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製品プロトコール

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ターゲットの情報

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Mikrochimica acta 192:131 PubMed39904887

2025

A turn-on fluorescent immunosensor for neurodegenerative disease related neurofilament light chain protein.

Applications

Unspecified application

Species

Unspecified reactive species

Qingting Song,Hailong Zhang,Jia Kong,Man Shing Wong,Hung-Wing Li

View all publications

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websiteProtocolBooklet

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Human NEFL ELISA Kit