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AB315307

Human NCAM1 ELISA Kit

Human NCAM1 ELISA Kit

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Human NCAM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NCAM1 with a sensitivity of 46.933 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

4 Images
Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)
  • sELISA

Supplier Data

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

Interpolated concentrations of human NCAM1 in SH-SY5Y cell culture supernatant

Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% SH-SY5Y cell culture supernatant.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)
  • sELISA

Supplier Data

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

Interpolated concentrations of NCAM1 in normal human serum donors

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 476.35 ng/mL with a range of 364.82 – 606.06 ng/mL.

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)
  • sELISA

Supplier Data

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples are 1% human serum, plasma – citrate, plasma – EDTA, plasma – heparin, and CSF. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)
  • sELISA

Supplier Data

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

Example of human NCAM1 standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

Quantitative
検出感度

= 46.933 pg/mL

検出範囲

78.125 - 5000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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組合せ製品

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human NCAM1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NCAM1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human NCAM1 with 46.933 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.4" } ]
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回収率

[ { "sample": "Serum", "range": "95 - 109 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "84 - 108 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "87 - 100 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "94 - 114 %", "average": "= 106" }, { "sample": "Cerebral Spinal Fluid", "range": "96 - 112 %", "average": "= 104" }, { "sample": "Cell culture supernatant", "range": "101 - 113 %", "average": "= 106" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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製品プロトコール

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ターゲットの情報

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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websiteProtocolBooklet
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