AB323605

Human NAD Kinase (NADK) ELISA Kit

Recombinant
SimpleStep
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Human NAD Kinase (NADK) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NAD Kinase (NADK) with a sensitivity of 27.8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

別名を表示する

NAD kinase, Poly(P)/ATP NAD kinase, NADK

4 Images

Sandwich ELISA - Human NAD Kinase (NADK) ELISA Kit (AB323605)

sELISA

Supplier Data

Sandwich ELISA - Human NAD Kinase (NADK) ELISA Kit (AB323605)

Example of human NAD Kinase standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NAD Kinase (NADK) ELISA Kit (AB323605)

Interpolated concentration of native NAD Kinase was measured in duplicate at different sample concentrations. Undiluted samples are 1,000 µg/mL HEK293T and 500 µg/mL MCF7 cell extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human NAD Kinase (NADK) ELISA Kit (AB323605)

Interpolated concentration of native NAD Kinase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL MCF7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human NAD Kinase (NADK) ELISA Kit (AB323605)

Example of human NAD Kinase standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Cell culture media, Cell Lysate

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 27.8 pg/mL

検出範囲

187.5 - 12000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human NAD Kinase (NADK) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NAD Kinase (NADK) protein in Serum, Citrate Plasma, EDTA Plasma, Cell culture media, Cell Lysate. Quantitate Human NAD Kinase (NADK) with 27.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.4" } ]

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回収率

[ { "sample": "Serum", "range": "88 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "84 - 85 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "87 - 89 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "100 - 100 %", "average": "= 100" }, { "sample": "Cell Lysate", "range": "89 - 95 %", "average": "= 93" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

See full target information NADK

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websiteProtocolBooklet

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Abcam product promise

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