AB278115

Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG)

Name: Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (ab278115) | Abcam
Brand: Abcam Limited
SKU: AB278115
Price: 102000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
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Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) is a single-wash 90-min Simplestep used to quantify Human Myelin oligodendrocyte glycoprotein (MOG) with a sensitivity of 1.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Myelin-oligodendrocyte glycoprotein, MOG

2 Images

Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)

sELISA

Supplier Data

Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)

Interpolated concentrations of native Myelin oligodendrocyte glycoprotein in human brain based on a 500 ng/mL extract load

sELISA

Supplier Data

Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)

Example of human Myelin oligodendrocyte glycoprotein standard curve in 1X Cell Extraction Buffer PTR.

The Myelin oligodendrocyte glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Adherent cells, Suspension cells, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 1.15 pg/mL

検出範囲

6.25 - 400 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human MOG ELISA kit (ab277115) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human MOG protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MOG with 1.15 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Myelin oligodendrocyte glycoprotein (MOG), a 16 kDa protein, mediates homophilic cell-cell adhesion. MOG is a component of the myelin sheath and may be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. Research indicates MOG acts as a receptor for the rubella virus. MOG shares 86%, 86%, 91%, and 98% sequence homology with mouse, rat, cow, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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回収率

[ { "sample": "Tissue Extracts", "range": "93 - 97 %", "average": "= 95" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278115-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278115-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278115-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278115-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278115-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278115-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278115-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Myelin oligodendrocyte glycoprotein (MOG) Capture Antibody", "number":"AB278115-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Myelin oligodendrocyte glycoprotein (MOG) Lyophilized Recombinant Protein", "number":"AB278115-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB278115-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB278115-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Myelin oligodendrocyte glycoprotein (MOG) Detector Antibody", "number":"AB278115-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myelin oligodendrocyte glycoprotein (MOG) also known as MOG protein or MOG glycoprotein is a lesser-known but important component of the central nervous system myelin. The MOG protein has a molecular mass of approximately 26 to 28 kDa. You will find it expressed on the surface of myelin sheaths and oligodendrocytes. This protein plays a role in the myelination process acting as a potential adhesive molecule or signaling molecule contributing to the stability and integrity of the myelin structure. Oligodendrocyte staining techniques can help visualize the distribution and expression patterns of MOG making it important for research purposes.

Biological function summary

MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.

Pathways

MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.

MOG plays a critical role in conditions such as multiple sclerosis and neuromyelitis optica. In multiple sclerosis MOG is a target of autoantibodies leading to demyelination and loss of neural function. In neuromyelitis optica MOG antibodies may contribute to severe inflammation and damage in optic nerves and spinal cord. The connection between MOG and these diseases highlights associations with other proteins like aquaporin-4 which are similarly targeted in associated autoimmune responses. Understanding these relationships is key for developing diagnostics or therapies involving MOG ELISA methods to detect MOG-specific antibodies in patient samples.

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製品プロトコール

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ターゲットの情報

Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.. (Microbial infection) Acts as a receptor for rubella virus.

See full target information MOG

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websiteProtocolBooklet

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Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG)